Human Recombinant NECTIN3 protein, His Tag (V2LY-0526-LY5712)

Name: Human Recombinant NECTIN3 protein, His Tag
SKU: V2LY-0526-LY5712
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant NECTIN3 protein, His Tag consist of Amino Acid: 1-400 and predicts a molecular mass of 39.3 kDa.

Molecule Mass

39.3 kDa

Sequence

Amino Acid: 1-400

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>98% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Nectin Cell Adhesion Molecule 3

Function

Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.

Biological Process

Establishment of protein localization to plasma membrane Source: ARUK-UCL
Fertilization Source: Ensembl
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: GO_Central
Homophilic cell adhesion via plasma membrane adhesion molecules Source: HGNC-UCL
Lens morphogenesis in camera-type eye Source: Ensembl
Protein localization to cell junction Source: Ensembl
Retina morphogenesis in camera-type eye Source: Ensembl
Spermatid development Source: InterPro

Cellular Location

Plasma membrane
Cell membrane
postsynaptic cell membrane

Topology

Extracellular: 58-404
Helical: 405-425
Cytoplasmic: 426-549

Zhao, Y., Hong, X. H., Li, K., Li, Y. Q., Li, Y. Q., He, S. W., ... & Chen, Y. P. (2020). ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin‐3 and NRXN3. Cancer Communications, 40(12), 721-737.

Hirabayashi, K., Tajiri, T., Bosch, D. E., Morimachi, M., Miyaoka, M., Inomoto, C., ... & Yeh, M. M. (2020). Loss of nectin‐3 expression as a marker of tumor aggressiveness in pancreatic neuroendocrine tumor. Pathology International, 70(2), 84-91.

Xu, F., Si, X., Wang, J., Yang, A., Qin, T., & Yang, Y. (2019). Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells. Biomedicine & Pharmacotherapy, 110, 139-144.

Liu, R., Wang, H., Wang, H. L., Sun, Y. X., Su, Y. A., Wang, X. D., ... & Si, T. M. (2019). Postnatal nectin‐3 knockdown induces structural abnormalities of hippocampal principal neurons and memory deficits in adult mice. Hippocampus, 29(11), 1063-1074.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant NECTIN3 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY5711)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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