Human Recombinant NPTN protein, ECD, His Tag (V2LY-0526-LY5829)

Name: Human Recombinant NPTN protein, ECD, His Tag
SKU: V2LY-0526-LY5829
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant NPTN protein, ECD, His Tag consist of Amino Acid: 1-221 and predicts a molecular mass of 23.2 kDa.

Molecule Mass

23.2 kDa

Protein Domain

ECD

Sequence

Amino Acid: 1-221

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

neuroplastin

Function

Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity (By similarity).
Also acts as a chaperone for ATP2B1; stabilizes ATP2B1 and increases its ATPase activity (PubMed:30190470).
Promotes localization of XKR8 at the cell membrane (PubMed:27503893).

Biological Process

Axon guidanceManual Assertion Based On ExperimentIBA:GO_Central
Cellular calcium ion homeostasisISS:UniProtKB
Dendrite self-avoidanceManual Assertion Based On ExperimentIBA:GO_Central
Excitatory synapse assemblyIEA:Ensembl
Homophilic cell adhesion via plasma membrane adhesion moleculesISS:HGNC-UCL
Long-term synaptic potentiationISS:UniProtKB
Negative regulation of cytokine productionISS:UniProtKB
Positive regulation of cytosolic calcium ion concentrationISS:UniProtKB
Positive regulation of ERK1 and ERK2 cascadeIEA:Ensembl
Positive regulation of fibroblast growth factor receptor signaling pathwayISS:UniProtKB
Positive regulation of long-term neuronal synaptic plasticityISS:HGNC-UCL
Positive regulation of long-term synaptic potentiationIEA:Ensembl
Positive regulation of neuron projection developmentISS:UniProtKB
Positive regulation of protein localizationIEA:Ensembl
Positive regulation of protein phosphorylationISS:UniProtKB
Regulation of receptor localization to synapseIEA:Ensembl
Trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmissionIEA:Ensembl
Visual learningIEA:Ensembl

Cellular Location

Cell membrane
Isoform 2
Cell junction, synapse, postsynaptic density

Topology

Extracellular: 29-339
Helical: 340-360
Cytoplasmic: 361-398

Li, S., Wei, X., Huang, H., Ye, L., Ma, M., Sun, L., ... & Wu, Y. (2023). Neuroplastin exerts antiepileptic effects through binding to the α1 subunit of GABA type A receptors to inhibit the internalization of the receptors. Journal of Translational Medicine, 21(1), 707.

Chen, J., Lin, X., Bhattacharya, S., Wiesehöfer, C., Wennemuth, G., Müller, K., & Montag, D. (2023). Neuroplastin Expression in Male Mice Is Essential for Fertility, Mating, and Adult Testosterone Levels. International Journal of Molecular Sciences, 25(1), 177.

Newton, S., Kong, F., Carlton, A. J., Aguilar, C., Parker, A., Codner, G. F., ... & Bowl, M. R. (2022). Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing. PLoS Genetics, 18(1), e1009937.

Ilic, K., Mlinac-Jerkovic, K., Sedmak, G., Rosenzweig, I., & Kalanj-Bognar, S. (2021). Neuroplastin in human cognition: review of literature and future perspectives. Translational Psychiatry, 11(1), 394.

Lin, X., Liang, Y., Herrera-Molina, R., & Montag, D. (2021). Neuroplastin in neuropsychiatric diseases. Genes, 12(10), 1507.

Lin, X., Brunk, M. G., Yuanxiang, P., Curran, A. W., Zhang, E., Stöber, F., ... & Montag, D. (2021). Neuroplastin expression is essential for hearing and hair cell PMCA expression. Brain Structure and Function, 226, 1533-1551.

Lin, X. (2021). Significance of neuroplastin and its paralog Basigin for plasma membrane-associated Ca 2+ ATPases in the central and peripheral nervous system.

Yagi, T., Asada, R., Kanekura, K., Eesmaa, A., Lindahl, M., Saarma, M., & Urano, F. (2020). Neuroplastin modulates anti-inflammatory effects of MANF. Iscience, 23(12).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human NPTN ELISA Kit (CAT#: V2LY-0626-LY4951)

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Isotype control

For research use only. Not intended for any clinical use.

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