Human Recombinant PBK protein, His Tag (V2LY-0526-LY5982)

Name: Human Recombinant PBK protein, His Tag
SKU: V2LY-0526-LY5982
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant PBK protein, His Tag consist of Amino Acid: 1-322 and predicts a molecular mass of 37 kDa.

Molecule Mass

37 kDa

Sequence

Amino Acid: 1-322

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>80% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

PDZ Binding Kinase

Function

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

Biological Process

Cellular response to UVIEA:Ensembl
Mitotic cell cycle1 PublicationNAS:UniProtKB
Negative regulation of inflammatory responseIEA:Ensembl
Negative regulation of proteasomal ubiquitin-dependent protein catabolic processIEA:Ensembl
Negative regulation of protein phosphorylationIEA:Ensembl
Negative regulation of stress-activated MAPK cascadeIEA:Ensembl

Cellular Location

Nucleus

PTM

Phosphorylated; in a cell-cycle dependent manner at mitosis.

Wu, H., Qian, J., Zhou, L., Hu, T., Zhang, Y., Wang, C., ... & Gu, C. (2024). FHND004 inhibits malignant proliferation of multiple myeloma by targeting PDZ-binding kinase in MAPK pathway. Aging (Albany NY), 16(5), 4811.

Mu, W., Xie, Y., Li, J., Yan, R., Zhang, J., Liu, Y. E., & Fan, Y. (2022). High expression of PDZ-binding kinase is correlated with poor prognosis and immune infiltrates in hepatocellular carcinoma. World Journal of Surgical Oncology, 20(1), 22.

Miki, Y., Devi, L., Imai, Y., Minami, N., Koide, T., & Goel, S. (2020). Deletion of the PDZ-binding kinase (Pbk) gene does not affect male fertility in mice. Reproduction, Fertility and Development, 32(10), 893-902.

Mao, P., Bao, G., Wang, Y. C., Du, C. W., Yu, X., Guo, X. Y., ... & Wang, M. D. (2020). PDZ-binding kinase-dependent transcriptional regulation of CCNB2 promotes tumorigenesis and radio-resistance in glioblastoma. Translational oncology, 13(2), 287-294.

Chen, D., Cox, J., Annam, J., Weingart, M., Essien, G., Rathi, K. S., ... & Schnepp, R. W. (2020). LIN28B promotes neuroblastoma metastasis and regulates PDZ binding kinase. Neoplasia, 22(6), 231-241.

Kar, A., Zhang, Y., Yacob, B. W., Saeed, J., Tompkins, K. D., Bagby, S. M., ... & Kiseljak-Vassiliades, K. (2019). Targeting PDZ-binding kinase is anti-tumorigenic in novel preclinical models of ACC. Endocrine-related cancer, 26(10), 765-778.

Warren, A. Y., Massie, C. E., Watt, K., Luko, K., Orafidiya, F., Selth, L. A., ... & Asim, M. (2019). A reciprocal feedback between the PDZ binding kinase and androgen receptor drives prostate cancer. Oncogene, 38(7), 1136-1150.

Zhang, Y., Yang, X., Wang, R., & Zhang, X. (2019). Prognostic value of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) in patients with cancer. Journal of Cancer, 10(1), 131.

Diao, X., Yang, D., Chen, Y., & Liu, W. (2019). Baicalin suppresses lung cancer growth by targeting PDZ-binding kinase/T-LAK cell-originated protein kinase. Bioscience Reports, 39(4), BSR20181692.

Kruthika, B. S., Jain, R., Arivazhagan, A., Bharath, R. D., Yasha, T. C., Kondaiah, P., & Santosh, V. (2019). Transcriptome profiling reveals PDZ binding kinase as a novel biomarker in peritumoral brain zone of glioblastoma. Journal of neuro-oncology, 141, 315-325.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PBK protein, GST Tag (CAT#: V2LY-0526-LY5980)

Human Recombinant PBK, Active protein, GST Tag (CAT#: V2LY-0526-LY5981)

PBK Matched Antibody Pair (845) (CAT#: APMAB-845LY)

Antibody Pairs

PBK Matched Antibody Pair (845) (CAT#: APMAB-845LY)

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Human Recombinant PBK, Active protein, GST Tag (CAT#: V2LY-0526-LY5981)

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Human Recombinant PBK protein, GST Tag (CAT#: V2LY-0526-LY5980)

Mouse Anti-PBK Recombinant Antibody (CBT4661) (CAT#: V2LY-0625-LY2699)

Mouse Anti-PBK Recombinant Antibody (1E11) (CAT#: CBMAB-A6369-LY)

PBK Matched Antibody Pair (845) (CAT#: APMAB-845LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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