Human Recombinant PCSK1 protein, His Tag (V2LY-0526-LY5991)

Name: Human Recombinant PCSK1 protein, His Tag
SKU: V2LY-0526-LY5991
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant PCSK1 protein, His Tag consist of Amino Acid: 1-617 and predicts a molecular mass of 57.4 kDa.

Molecule Mass

57.4 kDa

Sequence

Amino Acid: 1-617

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

proprotein convertase subtilisin/kexin type 1

Function

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.

Biological Process

Cell-cell signalingManual Assertion Based On ExperimentTAS:ProtInc
NeurogenesisIEA:Ensembl
Pancreas developmentIEA:Ensembl
Peptide biosynthetic processISS:BHF-UCL
Peptide hormone processingManual Assertion Based On ExperimentIBA:GO_Central
Pituitary gland developmentIEA:Ensembl
Positive regulation of protein secretionIEA:Ensembl
Protein autoprocessingIEA:Ensembl
Protein processingManual Assertion Based On ExperimentIBA:GO_Central
ProteolysisManual Assertion Based On ExperimentTAS:ProtInc
Response to axon injuryIEA:Ensembl
Response to calcium ionIEA:Ensembl
Response to chlorateIEA:Ensembl
Response to fatty acidIEA:Ensembl
Response to glucocorticoidIEA:Ensembl
Response to glucoseIEA:Ensembl
Response to interleukin-1IEA:Ensembl
Response to lipopolysaccharideIEA:Ensembl
Response to morphineIEA:Ensembl
Response to nutrient levelsIEA:Ensembl
Response to peptide hormoneIEA:Ensembl
Response to xenobiotic stimulusIEA:Ensembl

Cellular Location

Cytoplasmic vesicle, secretory vesicle
Localized in the secretion granules.

Involvement in disease

Proprotein convertase 1 deficiency (PC1 deficiency):
Characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.

PTM

O-glycosylated.

Folon, L., Baron, M., Toussaint, B., Vaillant, E., Boissel, M., Scherrer, V., ... & Bonnefond, A. (2023). Contribution of heterozygous PCSK1 variants to obesity and implications for precision medicine: a case-control study. The Lancet Diabetes & Endocrinology, 11(3), 182-190.

Shah, B. P., Sleiman, P. M., Mc Donald, J., Moeller, I. H., & Kleyn, P. (2023). Functional characterization of all missense variants in LEPR, PCSK1, and POMC genes arising from single-nucleotide variants. Expert Review of Endocrinology & Metabolism, 18(2), 209-219.

Van Dijck, E., Beckers, S., Diels, S., Huybrechts, T., Verrijken, A., Van Hoorenbeeck, K., ... & Van Hul, W. (2022). Rare heterozygous PCSK1 variants in human obesity: the contribution of the p. Y181H variant and a literature review. Genes, 13(10), 1746.

Shakya, M., Martin, N. K., Arunagiri, A., Martin, M. G., Arvan, P., Low, M. J., & Lindberg, I. (2022). The G209R mutant mouse as a model for human PCSK1 polyendocrinopathy. Endocrinology, 163(5), bqac024.

Duclaux-Loras, R., Bourgeois, P., Lavrut, P. M., Charbit-Henrion, F., Bonniaud-Blot, P., Maudinas, R., ... & Fabre, A. (2021). A novel mutation of PCSK1 responsible for PC1/3 deficiency in two siblings. Clinics and Research in Hepatology and Gastroenterology, 45(6), 101640.

Aerts, L., Terry, N. A., Sainath, N. N., Torres, C., Martín, M. G., Ramos-Molina, B., & Creemers, J. W. (2021). Novel homozygous inactivating mutation in the PCSK1 gene in an infant with congenital malabsorptive diarrhea. Genes, 12(5), 710.

Muhsin, N. I., Bentley, L., Bai, Y., Goldsworthy, M., & Cox, R. D. (2020). A novel mutation in the mouse Pcsk1 gene showing obesity and diabetes. Mammalian Genome, 31, 17-29.

Chou, C. L., Chen, T. J., Lin, C. Y., Lee, S. W., Wang, S. C., Chu, S. S., & Yang, C. C. (2020). PCSK1 overexpression in rectal cancer correlates with poor response to preoperative chemoradiotherapy and prognosis. OncoTargets and therapy, 3141-3150.

Jarvela, T. S., Shakya, M., Bachor, T., White, A., Low, M. J., & Lindberg, I. (2019). Reduced stability and pH-dependent activity of a common obesity-linked PCSK1 polymorphism, N221D. Endocrinology, 160(11), 2630-2645.

Pépin, L., Colin, E., Tessarech, M., Rouleau, S., Bouhours-Nouet, N., Bonneau, D., & Coutant, R. (2019). A new case of PCSK1 pathogenic variant with congenital proprotein convertase 1/3 deficiency and literature review. The Journal of clinical endocrinology & metabolism, 104(4), 985-993.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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