PCSK1 Antibodies

Structure of PCSK1

The molecular weight of the protein encoded by the PCSK1 gene is approximately 87 kDa, which slightly fluctuates among different mammals due to subtle differences in the precursor domain and catalytic region.

This protein belongs to the Subtilis protease family. Its primary structure contains approximately 753 amino acids and folds to form a typical trypsin-like double β -barrel catalytic domain. Its active center is composed of a catalytic triplet made up of Asp, His and Ser, which is responsible for specifically recognizing and cleave the paired basic amino acid sites of hormone precursors. The P domain of a protein is crucial for correct folding, transport, and the self-activation of zymogen in secretory granules, which serves as the structural basis for its hormone maturation function.

Fig. 1 PCSK1 structure.¹

Key structural properties of PCSK1:

• Typical Subtilis protease folds the double β -barrel catalytic domain
• Catalytic triplet active center composed of Asp, His and Ser
• P structure of key domain for enzyme folding, transport and the activation is crucial

Functions of PCSK1

The main function of the progen convertase 1 encoded by the PCSK1 gene is to specifically cleft hormone precursors in neuroendocrine cells to generate biologically active mature peptides. The physiological processes it participates in are extensive, covering energy metabolism, blood glucose stability and appetite regulation, etc.

The catalytic mechanism of this enzyme has high substrate specificity, and its activity is precisely regulated by intracellular pH, calcium ion concentration and the self-cleavage process of propeptides, ensuring that the hormone is activated and released at the correct time and place.

Applications of PCSK1 and PCSK1 Antibody in Literature

• Van Dijck, Evelien, et al. "Rare heterozygous PCSK1 variants in human obesity: the contribution of the p. Y181H variant and a literature review." Genes 13.10 (2022): 1746. https://doi.org/10.3390/genes13101746

Previous studies have suggested that the heterozygous variant p.Y181H of PCSK1 may increase the risk of obesity. This study compared obese people with those of normal weight and found that there was no significant difference in the carrying rate of this variant. Moreover, haplotype analysis suggested that it might be a rare pathogenic variant, and most missense variants had limited association with obesity.

• Ramos-Molina, Bruno, et al. "Hyperphagia and obesity in prader–willi syndrome: PCSK1 deficiency and beyond?." Genes 9.6 (2018): 288. https://doi.org/10.3390/genes9060288

This article reviews the partial overlapping mechanisms of overeating and obesity phenotypes between Prader-Willi syndrome and PCSK1 deficiency, and explores the potential therapeutic intervention approaches for both.

• Verde, Ludovica, et al. "The Interplay of UCP3 and PCSK1 Variants in Severe Obesity." Current Obesity Reports 14.1 (2025): 38. https://doi.org/10.1007/s13679-025-00631-1 

This study focuses on the association between rare variations in the UCP3 and PCSK1 genes and severe obesity. Two patients with early-onset obesity simultaneously carried p.Val192Ile and p.Asn221Asp variations, suggesting that multiple metabolic gene variations may synergistically exacerbate the obesity phenotype, which awaits verification through functional studies.

• Khan, Aleksandra Aljakna, et al. "Loss-of-function mutation in Pcsk1 increases serum APOA1 level and LCAT activity in mice." Laboratory Animal Research 38.1 (2022): 1. https://doi.org/10.1186/s42826-021-00111-2 

This study was the first to use a PCSK1-deficient mouse model to evaluate its role in high-density lipoprotein metabolism. It was found that the total and mature apolipoprotein A1 levels in the serum of mutant mice increased, but the HDL cholesterol concentration was not affected. The specific mechanism remains to be further clarified.

• Velazquez-Roman, Jorge, et al. "Association of PCSK1 and PPARG1 allelic variants with obesity and metabolic syndrome in Mexican adults." Genes 14.9 (2023): 1775. https://doi.org/10.3390/genes14091775 

This study found that PCSK1 gene variations were significantly associated with body mass index, waist-to-hip ratio, obesity and metabolic syndrome in the adult population in northwestern Mexico, suggesting that it may be an important genetic risk factor for metabolic diseases in this population.

Creative Biolabs: PCSK1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality PCSK1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom PCSK1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our PCSK1 antibodies, custom preparations, or technical support, contact us at email.