Human Recombinant SMYD2 protein, His Tag (V2LY-0526-LY6896)

Name: Human Recombinant SMYD2 protein, His Tag
SKU: V2LY-0526-LY6896
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant SMYD2 protein, His Tag consist of Amino Acid: 1-433 and predicts a molecular mass of 52 kDa.

Molecule Mass

52 kDa

Sequence

Amino Acid: 1-433

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

SET And MYND Domain Containing 2

Function

Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.

Biological Process

Biological Process chromatin organizationIEA:UniProtKB-KW
Biological Process heart developmentIEA:Ensembl
Biological Process histone lysine methylationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of cell population proliferationISS:UniProtKB
Biological Process negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process peptidyl-lysine dimethylationISS:UniProtKB
Biological Process peptidyl-lysine monomethylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of DNA damage response, signal transduction by p53 class mediatorManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of signal transduction by p53 class mediatorTAS:Reactome

Cellular Location

Cytoplasm, cytosol
Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

SMYD2 Matched Antibody Pair (1105) (CAT#: APMAB-1105LY)

Human Recombinant SMYD2, Active protein, GST Tag (CAT#: V2LY-0526-LY6895)

Antibody Pairs

SMYD2 Matched Antibody Pair (1105) (CAT#: APMAB-1105LY)

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Mouse Anti-SMYD2 Recombinant Antibody (14D3) (CAT#: CBMAB-0929-CN)

Rabbit Anti-SMYD2 Recombinant Antibody (CBXS-5337) (CAT#: CBMAB-S2550-CQ)

Mouse Anti-SMYD2 Recombinant Antibody (CBXS-0585) (CAT#: CBMAB-S3527-CQ)

Mouse Anti-SMYD2 Recombinant Antibody (CBXS-2519) (CAT#: CBMAB-S5276-CQ)

SMYD2 Matched Antibody Pair (1105) (CAT#: APMAB-1105LY)

Mouse Anti-SMYD2 Recombinant Antibody (CBXS-4152) (CAT#: CBMAB-S1405-CQ)

Rabbit Anti-SMYD2 Recombinant Antibody (CBXS-0584) (CAT#: CBMAB-S3526-CQ)

Human Recombinant SMYD2, Active protein, GST Tag (CAT#: V2LY-0526-LY6895)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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