Human SMYD2 ELISA Kit (V2LY-0626-LY4960)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0072 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
49.7 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
SET And MYND Domain Containing 2
Function
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.
Biological Process
Biological Process chromatin organizationIEA:UniProtKB-KW
Biological Process heart developmentIEA:Ensembl
Biological Process histone lysine methylationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of cell population proliferationISS:UniProtKB
Biological Process negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process peptidyl-lysine dimethylationISS:UniProtKB
Biological Process peptidyl-lysine monomethylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of DNA damage response, signal transduction by p53 class mediatorManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of signal transduction by p53 class mediatorTAS:Reactome
Cellular Location
Cytoplasm, cytosol
Nucleus
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

SMYD2 Matched Antibody Pair (1105) (CAT#: APMAB-1105LY)

Human Recombinant SMYD2, Active protein, GST Tag (CAT#: V2LY-0526-LY6895)

Human Recombinant SMYD2 protein, His Tag (CAT#: V2LY-0526-LY6896)

Antibody Pairs

SMYD2 Matched Antibody Pair (1105) (CAT#: APMAB-1105LY)

Related Products

Rabbit Anti-SMYD2 Recombinant Antibody (CBXS-0584) (CAT#: CBMAB-S3526-CQ)

Mouse Anti-SMYD2 Recombinant Antibody (CBXS-2519) (CAT#: CBMAB-S5276-CQ)

Rabbit Anti-SMYD2 Recombinant Antibody (CBXS-5337) (CAT#: CBMAB-S2550-CQ)

Mouse Anti-SMYD2 Recombinant Antibody (14D3) (CAT#: CBMAB-0929-CN)

Rabbit Anti-SMYD2 Recombinant Antibody (CBLY1-175) (CAT#: CBMAB-K2203-LY)

Mouse Anti-SMYD2 Recombinant Antibody (CBXS-0585) (CAT#: CBMAB-S3527-CQ)

Human Recombinant SMYD2, Active protein, GST Tag (CAT#: V2LY-0526-LY6895)

Mouse Anti-SMYD2 Recombinant Antibody (CBXS-2602) (CAT#: CBMAB-S5357-CQ)

Mouse Anti-SMYD2 Recombinant Antibody (CBT3550) (CAT#: V2LY-0625-LY1249)

SMYD2 Matched Antibody Pair (1105) (CAT#: APMAB-1105LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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