Human Recombinant TNFSF10 protein (V2LY-0526-LY7348)

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Datasheet
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Basic Information

Expressed Host
E. coli
Protein Species
Human
Protein Construction
This product is Human Recombinant TNFSF10 protein consist of Amino Acid: 114-281 and predicts a molecular mass of 19.6 kDa.
Molecule Mass
19.6 kDa
Sequence
Amino Acid: 114-281
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
≥95% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
TNFSF10
Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:26457518, PubMed:10549288).
Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Biological Process
Biological Process apoptotic process Source:ProtInc1 Publication
Biological Process cell-cell signaling Source:ProtInc1 Publication
Biological Process immune response Source:InterPro
Biological Process male gonad development Source:Ensembl
Biological Process positive regulation of apoptotic process Source:UniProtKB1 Publication
Biological Process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source:UniProtKB3 Publications
Biological Process positive regulation of extrinsic apoptotic signaling pathway Source:UniProtKB3 Publications
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signaling Source:UniProtKB1 Publication
Biological Process positive regulation of release of cytochrome c from mitochondria Source:UniProtKB1 Publication
Biological Process response to insulin Source:Ensembl
Biological Process signal transduction Source:ProtInc2 Publications
Cellular Location
Cell membrane
Secreted
Exists both as membrane-bound and soluble form.
Topology
Cytoplasmic: 1-17
Helical: 18-38
Extracellular: 39-281
PTM
Tyrosine phosphorylated by PKDCC/VLK.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-TNFSF10 Recombinant Antibody (CAT#: CBMAB-T3296-YJ)

Mouse Recombinant TNFSF10 protein, His Tag (CAT#: V2LY-0526-LY9007)

Rhesus Recombinant TNFSF10 protein (CAT#: V2LY-0526-LY9800)

Related Products

Mouse Anti-TNFSF10 Recombinant Antibody (CBFYC-3187) (CAT#: CBMAB-C3264-FY)

Rabbit Anti-TNFSF10 Recombinant Antibody (CBYJT-3900) (CAT#: CBMAB-T3298-YJ)

Mouse Anti-TNFSF10 Recombinant Antibody (SB91c) (CAT#: CBMAB-T3302-YJ)

Mouse Anti-TNFSF10 Recombinant Antibody (CBXS-0933) (CAT#: CBMAB-S3847-CQ)

Mouse Anti-TNFSF10 Recombinant Antibody (CBYJT-3897) (CAT#: CBMAB-T3292-YJ)

Rat Anti-Tnfsf10 Recombinant Antibody (CBYJT-3907) (CAT#: CBMAB-T3308-YJ)

Mouse Anti-TNFSF10 Antibody (11F2) (CAT#: CBMAB-1650CQ)

Rat Anti-Tnfsf10 Recombinant Antibody (N2B2) (CAT#: CBMAB-T3309-YJ)

Mouse Anti-TNFSF10 Recombinant Antibody (18) (CAT#: CBMAB-C3443-LY)

Mouse Anti-TNFSF10 Recombinant Antibody (RIK-2) (CAT#: CBMAB-C12589-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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