Human Recombinant YWHAH protein, GST Tag (V2LY-0526-LY1828)

Name: Human Recombinant YWHAH protein, GST Tag
SKU: V2LY-0526-LY1828
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant YWHAH protein, GST Tag consist of Amino Acid: 2-246 and predicts a molecular mass of 55 kDa.

Molecule Mass

55 kDa

Sequence

Amino Acid: 2-246

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥80% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

14-3-3 protein eta

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Biological Process

Biological Process glucocorticoid catabolic process Source:UniProtKB1 Publication
Biological Process glucocorticoid receptor signaling pathway Source:UniProtKB1 Publication
Biological Process intracellular protein transport Source:UniProtKB
Biological Process membrane depolarization during action potential Source:BHF-UCL1 Publication
Biological Process negative regulation of dendrite morphogenesis Source:UniProtKB
Biological Process positive regulation of DNA-templated transcription Source:UniProtKB1 Publication
Biological Process presynaptic modulation of chemical synaptic transmission Source:Ensembl
Biological Process protein localization Source:GO_Central1 Publication
Biological Process regulation of neuron differentiation Source:UniProtKB
Biological Process regulation of sodium ion transmembrane transporter activity Source:BHF-UCL1 Publication
Biological Process regulation of sodium ion transport Source:BHF-UCL1 Publication
Biological Process regulation of synaptic plasticity Source:UniProtKB
Biological Process signal transduction Source:GO_Central1 Publication
Biological Process substantia nigra development Source:UniProtKB1 Publication

Cellular Location

cerebellar granule cell to Purkinje cell synapse
cytoplasm
cytosol
extracellular exosome
intercalated disc
plasma membrane
presynapse

PTM

Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-YWHAH Recombinant Antibody (CAT#: CBMAB-BR497LY)

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Mouse Anti-YWHAH Recombinant Antibody (15j1) (CAT#: CBMAB-1237-LY)

Mouse Anti-YWHAH Recombinant Antibody (CAT#: CBMAB-BR497LY)

Mouse Anti-YWHAH Recombinant Antibody (CBLY2-110) (CAT#: CBMAB-1235-LY)

Mouse Anti-YWHAH Recombinant Antibody (13B811) (CAT#: CBMAB-1231-LY)

Mouse Anti-YWHAH Recombinant Antibody (3G12) (CAT#: CBMAB-0993CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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