Human WNT5B ELISA Kit (V2LY-0626-LY2879)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.05 ng/mL
Detection Range
0.1-30 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
40.3 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Wnt Family Member 5B
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Biological Process
Biological Process canonical Wnt signaling pathway Source:GO_Central1 Publication
Biological Process cell fate commitment Source:GO_Central1 Publication
Biological Process cellular response to retinoic acid Source:UniProtKB
Biological Process chondrocyte differentiation Source:UniProtKB1 Publication
Biological Process fat cell differentiation Source:UniProtKB1 Publication
Biological Process lens fiber cell development Source:BHF-UCLBy Similarity
Biological Process muscle cell differentiation Source:UniProtKB
Biological Process neuron differentiation Source:UniProtKB
Biological Process positive regulation of cell migration Source:UniProtKB1 Publication
Biological Process positive regulation of convergent extension involved in gastrulation Source:UniProtKB
Biological Process positive regulation of non-canonical Wnt signaling pathway Source:UniProtKB
Biological Process wound healing Source:Ensembl
Cellular Location
Secreted, extracellular space, extracellular matrix
PTM
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Wnt5b ELISA Kit (CAT#: V2LY-0626-LY2951)

Human WNT5B ELISA Kit (2) (CAT#: V2LY-0626-LY5122)

Related Products

Rat Anti-WNT5B Recombinant Antibody (CBWJW-191) (CAT#: CBMAB-W0078-WJ)

Rabbit Anti-WNT5B Recombinant Antibody (CBWJW-224) (CAT#: CBMAB-W0327-WJ)

Mouse Wnt5b ELISA Kit (CAT#: V2LY-0626-LY2951)

Mouse Anti-WNT5B Recombinant Antibody (3D1-F4-E9) (CAT#: CBMAB-W0094-WJ)

Human WNT5B ELISA Kit (2) (CAT#: V2LY-0626-LY5122)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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