Mouse Cd83 ELISA Kit (V2LY-0626-LY2146)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0024 ng/mL
Detection Range
0.005-1 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
21.3 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
CD83 Molecule
Function
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
Biological Process
Defense response Source: ProtInc
Humoral immune response Source: ProtInc
Negative regulation of interleukin-4 production Source: Ensembl
Positive regulation of CD4-positive, alpha-beta T cell differentiation Source: Ensembl
Positive regulation of interleukin-10 production Source: Ensembl
Positive regulation of interleukin-2 production Source: Ensembl
Response to organic cyclic compound Source: Ensembl
Signal transduction Source: ProtInc
Cellular Location
Membrane
Topology
Extracellular: 20-144
Helical: 145-166
Cytoplasmic: 167-205

Shrestha, A., Sadeyen, J. R., Lukosaityte, D., Chang, P., Smith, A., Van Hulten, M., & Iqbal, M. (2021). Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza. npj Vaccines, 6(1), 1-12.

Grosche, L., Knippertz, I., König, C., Royzman, D., Wild, A. B., Zinser, E., ... & Lechmann, M. (2020). The CD83 Molecule–An Important Immune Checkpoint. Frontiers in immunology, 11, 721.

Batool, A., Liu, H., Liu, Y. X., & Chen, S. R. (2020). CD83, a Novel MAPK Signaling Pathway Interactor, Determines Ovarian Cancer Cell Fate. Cancers, 12(8), 2269.

Islam, S. M., Byun, H. O., Choi, B., & Sohn, S. (2019). Inhibition of CD83 alleviates systemic inflammation in herpes simplex virus type 1-induced Behcet’s disease model mouse. Mediators of inflammation, 2019.

Zinser, E., Naumann, R., Wild, A. B., Michalski, J., Deinzer, A., Stich, L., ... & Knippertz, I. (2019). Endogenous expression of the human CD83 attenuates EAE symptoms in humanized transgenic mice and increases the activity of regulatory T cells. Frontiers in immunology, 10, 1442.

Nombela, I., Requena-Platek, R., Morales-Lange, B., Chico, V., Puente-Marin, S., Ciordia, S., ... & Ortega-Villaizan, M. D. M. (2019). Rainbow trout red blood cells exposed to viral hemorrhagic septicemia virus up-regulate antigen-processing mechanisms and MHC I&II, CD86, and CD83 antigen-presenting cell markers. Cells, 8(5), 386.

Wild, A. B., Krzyzak, L., Peckert, K., Stich, L., Kuhnt, C., Butterhof, A., ... & Steinkasserer, A. (2019). CD83 orchestrates immunity toward self and non-self in dendritic cells. JCI insight, 4(20).

Huo, S., Zhang, J., Wu, F., Zuo, Y., Cui, D., Li, X., ... & Zhong, F. (2019). Porcine CD83 is a glycosylated dimeric protein existing naturally in membrane-bound and soluble forms. Developmental & Comparative Immunology, 90, 60-69.

Lin, W., Buscher, K., Wang, B., Fan, Z., Song, N., Li, P., ... & Bi, H. (2018). Soluble CD83 alleviates experimental autoimmune uveitis by inhibiting filamentous actin-dependent calcium release in dendritic cells. Frontiers in immunology, 9, 1567.

Mo, Z. Q., Wang, J. L., Yang, M., Ni, L. Y., Wang, H. Q., Lao, G. F., ... & Dan, X. M. (2017). Characterization and expression analysis of grouper (Epinephelus coioides) co-stimulatory molecules CD83 and CD80/86 post Cryptocaryon irritans infection. Fish & shellfish immunology, 67, 467-474.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus, Rhesus Recombinant CD83 protein, hFc Tag (CAT#: V2LY-0526-LY1694)

Cynomolgus, Rhesus Recombinant CD83 protein, His Tag (CAT#: V2LY-0526-LY1695)

Human Recombinant CD83 protein, His Tag (CAT#: V2LY-0526-LY2967)

Mouse Recombinant CD83 protein, hFc Tag (CAT#: V2LY-0526-LY8076)

Mouse Recombinant CD83 protein, His Tag (CAT#: V2LY-0526-LY8077)

Rat Recombinant CD83 protein, hFc Tag (CAT#: V2LY-0526-LY9273)

Rat Recombinant CD83 protein, His Tag (CAT#: V2LY-0526-LY9274)

Human CD83 ELISA Kit (CAT#: V2LY-0626-LY5480)

Related Products

Rabbit Anti-CD83 Recombinant Antibody (CBFYC-1454) (CAT#: CBMAB-C1512-FY)

Mouse Anti-CD83 Recombinant Antibody (3G10-1F4) (CAT#: CBMAB-C6100-LY)

Mouse Recombinant CD83 protein, His Tag (CAT#: V2LY-0526-LY8077)

Rat Anti-CD83 Recombinant Antibody (4B5) (CAT#: CBMAB-C6667-LY)

Mouse Recombinant CD83 protein, hFc Tag (CAT#: V2LY-0526-LY8076)

Rat Recombinant CD83 protein, His Tag (CAT#: V2LY-0526-LY9274)

Mouse Anti-CD83 Recombinant Antibody (CBYY-C1601) (CAT#: CBMAB-C3039-YY)

Rabbit Anti-CD83 Recombinant Antibody (CP0230) (CAT#: CBMAB-CP0230-LY)

Cynomolgus, Rhesus Recombinant CD83 protein, His Tag (CAT#: V2LY-0526-LY1695)

Rat Recombinant CD83 protein, hFc Tag (CAT#: V2LY-0526-LY9273)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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