Mouse Chit1 ELISA Kit (V2LY-0626-LY2159)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0051 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
51.1 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Chitinase 1
Function
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
Biological Process
Chitin catabolic process Source: UniProtKB
Immune response Source: UniProtKB
Neutrophil degranulation Source: Reactome
Polysaccharide catabolic process Source: UniProtKB-KW
Polysaccharide digestion Source: Reactome
Response to bacterium Source: ProtInc
Cellular Location
Secreted; Lysosome. A small proportion is lysosomal.

Lee, J. H., Lee, C. M., Kim, M. O., Park, J. W., Kamle, S., Akosman, B., ... & Lee, C. G. (2021). Kasugamycin is a novel chitinase 1 inhibitor with strong antifibrotic effects on pulmonary fibrosis. bioRxiv.

Masrori, P., De Schaepdryver, M., Floeter, M. K., De Vocht, J., Lamaire, N., D'Hondt, A., ... & Van Damme, P. (2021). Prognostic relationship of neurofilaments, CHIT1, YKL-40 and MCP-1 in amyotrophic lateral sclerosis. Journal of Neurology, Neurosurgery & Psychiatry.

Lee, C. M., He, C. H., Park, J. W., Lee, J. H., Kamle, S., Ma, B., ... & Lee, C. G. (2019). Chitinase 1 regulates pulmonary fibrosis by modulating TGF-β/SMAD7 pathway via TGFBRAP1 and FOXO3. Life science alliance, 2(3).

Logue, E. C., Neff, C. P., Mack, D. G., Martin, A. K., Fiorillo, S., Lavelle, J., ... & Fontenot, A. P. (2019). Upregulation of chitinase 1 in alveolar macrophages of HIV-infected smokers. The Journal of Immunology, 202(5), 1363-1372.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CHIT1 protein, His Tag (CAT#: V2LY-0526-LY1468)

Human Recombinant CHIT1 protein, His Tag (CAT#: V2LY-0526-LY3119)

Mouse Recombinant CHIT1 protein, His Tag (CAT#: V2LY-0526-LY8105)

Related Products

Mouse Anti-CHIT1 Recombinant Antibody (CBFYC-1838) (CAT#: CBMAB-C1902-FY)

Mouse Anti-CHIT1 Recombinant Antibody (CBXC-1172) (CAT#: CBMAB-C5346-CQ)

Mouse Anti-CHIT1 Recombinant Antibody (CBYY-C0056) (CAT#: CBMAB-C0175-YY)

Mouse Recombinant CHIT1 protein, His Tag (CAT#: V2LY-0526-LY8105)

Mouse Anti-CHIT1 Recombinant Antibody (CBT337) (CAT#: V2LY-0625-LY3586)

Mouse Anti-CHIT1 Recombinant Antibody (CBYY-C2056) (CAT#: CBMAB-C3494-YY)

Human Recombinant CHIT1 protein, His Tag (CAT#: V2LY-0526-LY3119)

Mouse Anti-CHIT1 Recombinant Antibody (AO312) (CAT#: CBMAB-AO312LY)

Cynomolgus Recombinant CHIT1 protein, His Tag (CAT#: V2LY-0526-LY1468)

Rabbit Anti-CHIT1 Recombinant Antibody (EN815) (CAT#: CBMAB-EN815-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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