Mouse Cma1 ELISA Kit (V2LY-0626-LY2170)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.026 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
27.6 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Chymase 1
Function
Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Biological Process
Angiotensin maturation Source: Reactome
Basement membrane disassembly Source: BHF-UCL
Cellular response to glucose stimulus Source: Ensembl
Cytokine precursor processing Source: BHF-UCL
Extracellular matrix disassembly Source: BHF-UCL
Midbrain development Source: Ensembl
Positive regulation of angiogenesis Source: Ensembl
Regulation of inflammatory response Source: BHF-UCL
Cellular Location
Secreted; Cytoplasmic granule. Mast cell granules.

Ferrario, C. M., Groban, L., Wang, H., Cheng, C. P., VonCannon, J. L., Wright, K. N., ... & Ahmad, S. (2021). The Angiotensin-(1–12)/Chymase axis as an alternate component of the tissue renin angiotensin system. Molecular and Cellular Endocrinology, 529, 111119.

Shi, S., Ye, S., Mao, J., Ru, Y., Lu, Y., Wu, X., ... & Xi, Y. (2020). CMA1 is potent prognostic marker and associates with immune infiltration in gastric cancer. Autoimmunity, 53(4), 210-217.

Zhou, D., Chen, Y., Wu, J., Shen, J., Shang, Y., Zheng, L., & Xie, X. (2019). Association between chymase gene polymorphisms and atrial fibrillation in Chinese Han population. BMC Cardiovascular Disorders, 19(1), 1-5.

Sabbah, H. N., Gupta, R. C., Singh-Gupta, V., & Zhang, K. (2019). LONG-TERM THERAPY BAY 1142524, A CHYMASE-1 INHIBITOR, NORMALIZES PLASMA BIOMARKERS IN DOGS WITH CORONARY MICROEMBOLIZATION-INDUCED HEART FAILURE. Journal of the American College of Cardiology, 73(9S1), 699-699.

Varney, V. A., Nicholas, A., Warner, A., & Sumar, N. (2019). IgE-mediated systemic anaphylaxis and its association with gene polymorphisms of ACE, angiotensinogen and chymase. Journal of Asthma and Allergy, 12, 343.

Meyer, N., Woidacki, K., Knöfler, M., Meinhardt, G., Nowak, D., Velicky, P., ... & Zenclussen, A. C. (2017). Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth. Scientific reports, 7(1), 1-14.

Sabbah, H. N., Gupta, R. C., & Tinel, H. (2017). Long-term therapy with a chymase-1 inhibitor (BAY 1142524) improves left ventricular systolic function and prevents progressive chamber remodeling in dogs with heart failure. Circulation, 136(suppl_1), A11585-A11585.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CMA1 protein, His Tag (CAT#: V2LY-0526-LY3136)

Human CMA1 ELISA Kit (CAT#: V2LY-0626-LY4861)

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Mouse Anti-CMA1 Recombinant Antibody (C1278) (CAT#: V2LY-1206-LY338)

Mouse Anti-CMA1 Recombinant Antibody (CBFYM-1016) (CAT#: V2LY-1206-LY341)

Human CMA1 ELISA Kit (CAT#: V2LY-0626-LY4861)

Human Recombinant CMA1 protein, His Tag (CAT#: V2LY-0526-LY3136)

Mouse Anti-CMA1 Recombinant Antibody (CBYY-C0217) (CAT#: CBMAB-C0624-YY)

Rabbit Anti-CMA1 Recombinant Antibody (CBFYM-1015) (CAT#: CBMAB-M1167-FY)

Mouse Anti-CMA1 Recombinant Antibody (C4685) (CAT#: V2LY-1206-LY345)

Isotype control

For research use only. Not intended for any clinical use.

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