Mouse Dbi ELISA Kit (V2LY-0626-LY1990)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.29 ng/mL
Detection Range
0.5-200 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
10 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Diazepam Binding Inhibitor, Acyl-CoA Binding Protein
Function
Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
Biological Process
Acyl-CoA metabolic process Source: Reactome
Negative regulation of protein lipidation Source: UniProtKB
Phosphatidylcholine acyl-chain remodeling Source: UniProtKB
Positive regulation of CoA-transferase activity Source: UniProtKB
Positive regulation of phospholipid transport Source: UniProtKB
Cellular Location
Golgi apparatus; Endoplasmic reticulum. Golgi localization is dependent on ligand binding (PubMed:17953517).

Joseph, A., Chen, H., Anagnostopoulos, G., Montégut, L., Lafarge, A., Motiño, O., ... & Kroemer, G. (2021). Effects of acyl-coenzyme A binding protein (ACBP)/diazepam-binding inhibitor (DBI) on body mass index. Cell death & disease, 12(6), 1-14.

Thomas, A. M., Asnicar, F., Kroemer, G., & Segata, N. (2021). Genes Encoding Microbial Acyl Coenzyme A Binding Protein/Diazepam-Binding Inhibitor Orthologs Are Rare in the Human Gut Microbiome and Show No Links to Obesity. Applied and environmental microbiology, 87(12), e00471-21.

Mamoor, S. (2021). Over-expression of diazepam binding inhibitor, acyl-CoA binding protein in human endometrial cancer.

Alquier, T., Christian-Hinman, C. A., Alfonso, J., & Færgeman, N. J. (2021). From benzodiazepines to fatty acids and beyond: revisiting the role of ACBP/DBI. Trends in Endocrinology & Metabolism, 32(11), 890-903.

Joseph, A., Moriceau, S., Sica, V., Anagnostopoulos, G., Pol, J., Martins, I., ... & Kroemer, G. (2020). Metabolic and psychiatric effects of acyl coenzyme A binding protein (ACBP)/diazepam binding inhibitor (DBI). Cell death & disease, 11(7), 1-10.

Charmpilas, N., Ruckenstuhl, C., Sica, V., Büttner, S., Habernig, L., Dichtinger, S., ... & Kroemer, G. (2020). Acyl-CoA-binding protein (ACBP): a phylogenetically conserved appetite stimulator. Cell death & disease, 11(1), 1-10.

Bravo-San Pedro, J. M., Sica, V., Martins, I., Anagnostopoulos, G., Maiuri, C., & Kroemer, G. (2019). Cell-autonomous, paracrine and neuroendocrine feedback regulation of autophagy by DBI/ACBP (diazepam binding inhibitor, acyl-CoA binding protein): the obesity factor. Autophagy, 15(11), 2036-2038.

Bravo-San Pedro, J. M., Sica, V., Madeo, F., & Kroemer, G. (2019). Acyl-CoA-binding protein (ACBP): the elusive ‘hunger factor'linking autophagy to food intake. Cell Stress, 3(10), 312.

Bravo-San Pedro, J. M., Sica, V., Martins, I., Pol, J., Loos, F., Maiuri, M. C., ... & Kroemer, G. (2019). Acyl-CoA-binding protein is a lipogenic factor that triggers food intake and obesity. Cell metabolism, 30(4), 754-767.

Duman, C., Yaqubi, K., Hoffmann, A., Acikgöz, A. A., Korshunov, A., Bendszus, M., ... & Alfonso, J. (2019). Acyl-CoA-binding protein drives glioblastoma tumorigenesis by sustaining fatty acid oxidation. Cell metabolism, 30(2), 274-289.

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For research use only. Not intended for any clinical use.

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