Mouse Nell1 ELISA Kit (V2LY-0626-LY2929)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.019 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
89.4 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Neural EGFL Like 1
Function
Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization.
Biological Process
Cell differentiation Source: UniProtKB-KW
Negative regulation of cellular protein catabolic process Source: UniProtKB
Negative regulation of osteoblast proliferation Source: UniProtKB
Nervous system development Source: ProtInc
Positive regulation of bone mineralization Source: UniProtKB
Positive regulation of osteoblast differentiation Source: UniProtKB
Regulation of gene expression Source: UniProtKB
Cellular Location
Nucleus
Nucleus envelope
Cytoplasm
Secreted
Note: Colocalizes with ATRAID on the nuclear envelope and the perinuclear region.

Sethi, S. (2023). The many faces of NELL1 MN. Clinical Kidney Journal, 16(3), 442-446.

Kurien, A. A., Ks, J. P., Walker, P. D., & Caza, T. N. (2022). Traditional indigenous medicines are an etiologic consideration for NELL1-positive membranous nephropathy. Kidney International, 102(6), 1424-1426.

Cheng, X., Shi, J., Jia, Z., Ha, P., Soo, C., Ting, K., ... & Zhang, X. (2022). NELL-1 in genome-wide association studies across human diseases. The American Journal of Pathology, 192(3), 395-405.

Qin, Q., Gomez-Salazar, M., Tower, R. J., Chang, L., Morris, C. D., McCarthy, E. F., ... & James, A. W. (2022). NELL1 regulates the matrisome to promote osteosarcoma progression. Cancer research, 82(15), 2734-2747.

Duan, C., & Townley, H. E. (2022). Isolation of NELL 1 Aptamers for Rhabdomyosarcoma Targeting. Bioengineering, 9(4), 174.

Spain, R. I., Andeen, N. K., Gibson, P. C., Samuels, M. H., Morris, C. D., Solomon, A. J., ... & Avasare, R. S. (2021). Lipoic acid supplementation associated with neural epidermal growth factor-like 1 (NELL1)–associated membranous nephropathy. Kidney International, 100(6), 1208-1213.

Caza, T. N., Hassen, S. I., Dvanajscak, Z., Kuperman, M., Edmondson, R., Herzog, C., ... & Larsen, C. P. (2021). NELL1 is a target antigen in malignancy-associated membranous nephropathy. Kidney international, 99(4), 967-976.

Sethi, S., Debiec, H., Madden, B., Charlesworth, M. C., Morelle, J., Gross, L., ... & Ronco, P. (2020). Neural epidermal growth factor-like 1 protein (NELL-1) associated membranous nephropathy. Kidney International, 97(1), 163-174.

Huang, X., Cen, X., Zhang, B., Liao, Y., Zhao, Z., Zhu, G., ... & Liu, J. (2019). The roles of circRFWD2 and circINO80 during NELL‐1‐induced osteogenesis. Journal of Cellular and Molecular Medicine, 23(12), 8432-8441.

Li, C., Zhang, X., Zheng, Z., Nguyen, A., Ting, K., & Soo, C. (2019). Nell-1 is a key functional modulator in osteochondrogenesis and beyond. Journal of Dental Research, 98(13), 1458-1468.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human NELL1 ELISA Kit (CAT#: V2LY-0626-LY5104)

NELL1 Matched Antibody Pair (792) (CAT#: APMAB-792LY)

Antibody Pairs

NELL1 Matched Antibody Pair (792) (CAT#: APMAB-792LY)

Related Products

Human NELL1 ELISA Kit (CAT#: V2LY-0626-LY5104)

Mouse Anti-NELL1 Recombinant Antibody (CBWJN-1324) (CAT#: CBMAB-N1955-WJ)

Mouse Anti-NELL1 Recombinant Antibody (CBWJN-1323) (CAT#: CBMAB-N1954-WJ)

NELL1 Matched Antibody Pair (792) (CAT#: APMAB-792LY)

Mouse Anti-NELL1 Recombinant Antibody (CBWJN-0506) (CAT#: CBMAB-N1960-WJ)

Mouse Anti-NELL1 Recombinant Antibody (6A8) (CAT#: CBMAB-N1959-WJ)

Mouse Anti-NELL1 Recombinant Antibody (3F1) (CAT#: CBMAB-N1958-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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