Mouse Recombinant BACE2 protein, His Tag (V2LY-0526-LY7854)

Name: Mouse Recombinant BACE2 protein, His Tag
SKU: V2LY-0526-LY7854
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant BACE2 protein, His Tag consist of Amino Acid: 1-462 and predicts a molecular mass of 49.2 kDa.

Molecule Mass

49.2 kDa

Sequence

Amino Acid: 1-462

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, CaCl2, Arginine

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Beta-Site APP-Cleaving Enzyme 2

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Responsible also for the proteolytic processing of CLTRN in pancreatic beta cells (PubMed:21907142).

Biological Process

Amyloid-beta metabolic process Source: GO_Central
Astrocyte activation Source: Ensembl
Glucose homeostasis Source: UniProtKB
Membrane protein ectodomain proteolysis Source: UniProtKB
Negative regulation of amyloid precursor protein biosynthetic process Source: UniProtKB
Peptide hormone processing Source: UniProtKB
Proteolysis Source: UniProtKB

Cellular Location

Endoplasmic reticulum; Golgi apparatus; Endosome; Cell membrane

Topology

Extracellular: 21-473 aa
Helical: 474-494 aa
Cytoplasmic: 495-518 aa

PTM

Undergoes autoproteolytic cleavage.
Glycosylated.

Fujimoto, K., Yoshida, S., Tadano, G., Asada, N., Fuchino, K., Suzuki, S., ... & Kusakabe, K. I. (2021). Structure-Based Approaches to Improving Selectivity through Utilizing Explicit Water Molecules: Discovery of Selective β-Secretase (BACE1) Inhibitors over BACE2. Journal of Medicinal Chemistry, 64(6), 3075-3085.

He, F., Yu, J., Yang, J., Wang, S., Zhuang, A., Shi, H., ... & Jia, R. (2021). m6A RNA hypermethylation-induced BACE2 boosts intracellular calcium release and accelerates tumorigenesis of ocular melanoma. Molecular Therapy.

Yen, Y. C., Kammeyer, A. M., Tirlangi, J., Ghosh, A. K., & Mesecar, A. D. (2021). A Structure-Based Discovery Platform for BACE2 and the Development of Selective BACE Inhibitors. ACS Chemical Neuroscience, 12(4), 581-588.

Wang, Z., Xu, Q., Cai, F., Liu, X., Wu, Y., & Song, W. (2019). BACE2, a conditional β-secretase, contributes to Alzheimer’s disease pathogenesis. JCI insight, 4(1).

Liu, F., Zhang, Y., Liang, Z., Sun, Q., Liu, H., Zhao, J., ... & Sun, X. (2018). Cleavage of potassium channel Kv2. 1 by BACE2 reduces neuronal apoptosis. Molecular psychiatry, 23(7), 1542-1554.

Alcarraz-Vizán, G., Castaño, C., Visa, M., Montane, J., Servitja, J. M., & Novials, A. (2017). BACE2 suppression promotes β-cell survival and function in a model of type 2 diabetes induced by human islet amyloid polypeptide overexpression. Cellular and Molecular Life Sciences, 74(15), 2827-2838.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant BACE2 protein, His Tag (CAT#: V2LY-0526-LY2284)

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Human Recombinant BACE2 protein, His Tag (CAT#: V2LY-0526-LY2284)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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