Mouse Recombinant PPM1A protein, His Tag (V2LY-0526-LY8796)

Name: Mouse Recombinant PPM1A protein, His Tag
SKU: V2LY-0526-LY8796
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant PPM1A protein, His Tag consist of Amino Acid: 1-382 and predicts a molecular mass of 43.3 kDa.

Molecule Mass

43.3 kDa

Sequence

Amino Acid: 1-382

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>98% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform

Function

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.

Biological Process

Cellular response to transforming growth factor beta stimulusIEA:Ensembl
DephosphorylationManual Assertion Based On ExperimentIDA:BHF-UCL
N-terminal protein myristoylationISS:UniProtKB
Negative regulation of BMP signaling pathwayIEA:Ensembl
Negative regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of SMAD protein complex assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IITAS:Reactome
Negative regulation of transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-threonine dephosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentHMP:UniProtKB
Positive regulation of protein export from nucleusIEA:Ensembl
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:BHF-UCL
Protein dephosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Protein export from nucleusIEA:Ensembl
Regulation of cell cycleTAS:Reactome

Cellular Location

Nucleus
Cytoplasm, cytosol
Membrane
Weakly associates at the membrane and N-myristoylation mediates the membrane localization.

PTM

N-myristoylation is essential for the recognition of its substrates for dephosphorylation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PPM1A, Active protein, GST Tag (CAT#: V2LY-0526-LY6264)

Human Recombinant PPM1A, Active protein, GST Tag-1 (CAT#: V2LY-0526-LY6265)

Human Recombinant PPM1A protein, His Tag (CAT#: V2LY-0526-LY6266)

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Mouse Anti-PPM1A Recombinant Antibody (13B635) (CAT#: CBMAB-P2550-YC)

Mouse Anti-PPM1A Recombinant Antibody (4E11) (CAT#: CBMAB-P2551-YC)

Mouse Anti-PPM1A Recombinant Antibody (11C45) (CAT#: CBMAB-P2549-YC)

Mouse Anti-PPM1A Recombinant Antibody (7F12) (CAT#: CBMAB-P2552-YC)

Human Recombinant PPM1A, Active protein, GST Tag (CAT#: V2LY-0526-LY6264)

Mouse Anti-PPM1A Recombinant Antibody (CBT2944) (CAT#: V2LY-0625-LY2752)

Human Recombinant PPM1A protein, His Tag (CAT#: V2LY-0526-LY6266)

Human Recombinant PPM1A, Active protein, GST Tag-1 (CAT#: V2LY-0526-LY6265)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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