Mouse Wnt8a ELISA Kit (V2LY-0626-LY2956)

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Tested Data
Request for COA
Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.011 ng/mL
Detection Range
0.02-7 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
39.6 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Wnt family member 8A
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in embryonic patterning.
Biological Process
Biological Process anterior/posterior axis specification Source:ARUK-UCL
Biological Process canonical Wnt signaling pathway Source:WormBase1 Publication
Biological Process canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment Source:BHF-UCL1 Publication
Biological Process canonical Wnt signaling pathway involved in neural crest cell differentiation Source:BHF-UCL
Biological Process cell fate commitment Source:GO_Central1 Publication
Biological Process neural crest cell fate commitment Source:BHF-UCL
Biological Process neuron differentiation Source:GO_Central1 Publication
Biological Process positive regulation of transcription by RNA polymerase II Source:BHF-UCL
Biological Process response to retinoic acid Source:BHF-UCL1 Publication
Biological Process secondary palate development Source:BHF-UCL1 Publication
Cellular Location
Secreted, extracellular space, extracellular matrix
Secreted
PTM
Palmitoleoylation is required for efficient binding to frizzled receptors (By similarity).
Depalmitoleoylation leads to Wnt signaling pathway inhibition (By similarity).
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT8A.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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