Mouse Wnt8b ELISA Kit (V2LY-0626-LY2957)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0089 ng/mL
Detection Range
0.02-3.8 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
38.6 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Wnt Family Member 8B
Function
Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Biological Process
Biological Process canonical Wnt signaling pathway Source:WormBase1 Publication
Biological Process cell fate commitment Source:GO_Central1 Publication
Biological Process cellular response to retinoic acid Source:UniProtKB
Biological Process determination of dorsal identity Source:BHF-UCLBy Similarity
Biological Process gastrulation Source:BHF-UCLBy Similarity
Biological Process nervous system development Source:ProtInc1 Publication
Biological Process neuron differentiation Source:UniProtKB
Biological Process response to estradiol Source:BHF-UCL1 Publication
Biological Process response to retinoic acid Source:BHF-UCL1 Publication
Biological Process signal transduction Source:ProtInc1 Publication
Cellular Location
Secreted, extracellular space, extracellular matrix
PTM
Palmitoleoylation is required for efficient binding to frizzled receptors (By similarity).
Depalmitoleoylation leads to Wnt signaling pathway inhibition (By similarity).
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT8B.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Rat Anti-WNT8B Recombinant Antibody (CBWJW-115) (CAT#: CBMAB-W0264-WJ)

Rat Anti-WNT8B Recombinant Antibody (CBWJW-073) (CAT#: CBMAB-W0227-WJ)

Rat Anti-WNT8B Recombinant Antibody (CBWJW-074) (CAT#: CBMAB-W0228-WJ)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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