Rat Jak1 ELISA Kit (V2LY-0626-LY13)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.14 ng/mL
Detection Range
0.25-16 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
133.3 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Janus kinase 1
Alternative Names
JAK 1,JAK1,JAK1A,JAK1B,Janus kinase 1,JTK3,Tyrosine protein kinase JAK1
Function
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:7615558, PubMed:28111307, PubMed:32750333, PubMed:16239216).
Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529) as well as interleukin (IL)-10 receptor (PubMed:12133952).
Directly phosphorylates STAT but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors (PubMed:16239216, PubMed:32750333).
Biological Process
Cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Interferon-gamma-mediated signaling pathwayTAS:Reactome
Interleukin-2-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Interleukin-6-mediated signaling pathwayTAS:Reactome
Intracellular signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of homotypic cell-cell adhesionManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of sprouting angiogenesisManual Assertion Based On ExperimentIGI:BHF-UCL
Protein localization to cell-cell junctionManual Assertion Based On ExperimentIMP:ARUK-UCL
Protein phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Receptor signaling pathway via JAK-STATManual Assertion Based On ExperimentIMP:UniProtKB
Response to antibioticManual Assertion Based On ExperimentIDA:MGI
Type I interferon signaling pathwayTAS:Reactome
Cellular Location
Endomembrane system. Wholly intracellular, possibly membrane associated.
Involvement in disease
Autoinflammation, immune dysregulation, and eosinophilia (AIIDE):
An autosomal dominant disorder characterized by immune dysregulation, severe atopic dermatitis, and chronic gastrointestinal inflammation. Additional features include asthma, food or environmental allergies, as well as poor overall growth with short stature.
PTM
Autophosphorylated (PubMed:7615558).
Phosphorylated on tyrosine residues in response to interferon gamma signaling (PubMed:7615558).
Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling (PubMed:11909529).
Ubiquitinated by RNF125; leading to its degradation by the proteasome.

Chapman, S., Kwa, M., Gold, L. S., & Lim, H. W. (2022). Janus kinase inhibitors in dermatology: Part I. A comprehensive review. Journal of the American Academy of Dermatology, 86(2), 406-413.

Roskoski Jr, R. (2022). Janus kinase (JAK) inhibitors in the treatment of neoplastic and inflammatory disorders. Pharmacological Research, 106362.

Spinelli, F. R., Colbert, R. A., & Gadina, M. (2021). JAK1: Number one in the family; number one in inflammation?. Rheumatology, 60(Supplement_2), ii3-ii10.

Fetter, T., Smith, P., Guel, T., Braegelmann, C., Bieber, T., & Wenzel, J. (2020). Selective janus kinase 1 inhibition is a promising therapeutic approach for lupus erythematosus skin lesions. Frontiers in Immunology, 11, 344.

Choy, E. H. (2019). Clinical significance of Janus Kinase inhibitor selectivity. Rheumatology, 58(6), 953-962.

Zak, M., Hanan, E. J., Lupardus, P., Brown, D. G., Robinson, C., Siu, M., ... & Johnson, A. (2019). Discovery of a class of highly potent Janus kinase 1/2 (JAK1/2) inhibitors demonstrating effective cell-based blockade of IL-13 signaling. Bioorganic & medicinal chemistry letters, 29(12), 1522-1531.

Papadopoulou, C., Hong, Y., Omoyinmi, E., Brogan, P. A., & Eleftheriou, D. (2019). Janus kinase 1/2 inhibition with baricitinib in the treatment of juvenile dermatomyositis. Brain, 142(3), e8-e8.

Abdou, A. G., Maraee, A., Yassien, H., & Sarhan, M. (2018). Immunohistochemistry of Janus kinase 1 (JAK1) expression in vitiligo. Journal of Pathology and Translational Medicine, 52(6), 363-368.

Jarocha, D. J., Gadue, P., Tong, W., Newton, R. C., & Poncz, M. (2018). Janus kinase (JAK) 1 inhibition affects both megakaryopoiesis and thrombopoiesis. Blood, 132, 2559.

Wehde, B. L., Rädler, P. D., Shrestha, H., Johnson, S. J., Triplett, A. A., & Wagner, K. U. (2018). Janus kinase 1 plays a critical role in mammary cancer progression. Cell reports, 25(8), 2192-2207.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human JAK1 ELISA Kit (CAT#: V2LY-0626-LY4090)

Human Recombinant JAK1 (d966-989) protein, GST Tag (CAT#: V2LY-0526-LY5093)

Human Recombinant JAK1 (E966V) protein, GST Tag (CAT#: V2LY-0526-LY5094)

Human Recombinant JAK1 protein, GST Tag (CAT#: V2LY-0526-LY5095)

Human Recombinant JAK1, Active protein, GST Tag (CAT#: V2LY-0526-LY5096)

Human Recombinant JAK1 (JH1), Active protein, GST Tag (CAT#: V2LY-0526-LY5097)

Human Recombinant JAK1 (R724H), Active protein, GST Tag (CAT#: V2LY-0526-LY5098)

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For research use only. Not intended for any clinical use.

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