Rat Ldha ELISA Kit (V2LY-0626-LY2984)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.024 mU/mL
Detection Range
0.05-30 mU/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
36.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
LDHA
Biological Process
Glycolytic process1 PublicationNAS:UniProtKB
Lactate metabolic processManual Assertion Based On ExperimentIDA:ComplexPortal
Pyruvate metabolic processManual Assertion Based On ExperimentIDA:ComplexPortal
Substantia nigra developmentHEP:UniProtKB
Cellular Location
Cytoplasm
Involvement in disease
Glycogen storage disease 11 (GSD11):
A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
PTM
ISGylated.

Ban, E. J., Kim, D., Kim, J. K., Kang, S. W., Lee, J., Jeong, J. J., ... & Kim, K. (2021). Lactate dehydrogenase A as a potential new biomarker for thyroid cancer. Endocrinology and Metabolism, 36(1), 96-105.

Dai, C., Li, Q., May, H. I., Li, C., Zhang, G., Sharma, G., ... & Wang, Z. V. (2020). Lactate dehydrogenase A governs cardiac hypertrophic growth in response to hemodynamic stress. Cell reports, 32(9).

Yu, H., Yin, Y., Yi, Y., Cheng, Z., Kuang, W., Li, R., ... & Zhang, G. (2020). Targeting lactate dehydrogenase A (LDHA) exerts antileukemic effects on T‐cell acute lymphoblastic leukemia. Cancer Communications, 40(10), 501-517.

Hong, M., Zhang, X. B., Xiang, F., Fei, X., Ouyang, X. L., & Peng, X. C. (2020). MiR-34a suppresses osteoblast differentiation through glycolysis inhibition by targeting lactate dehydrogenase-A (LDHA). In Vitro Cellular & Developmental Biology-Animal, 56, 480-487.

Woodford, M. R., Chen, V. Z., Backe, S. J., Bratslavsky, G., & Mollapour, M. (2019). Structural and functional regulation of lactate dehydrogenase-A in cancer. Future Medicinal Chemistry, 12(5), 439-455.

Jafary, F., Ganjalikhany, M. R., Moradi, A., Hemati, M., & Jafari, S. (2019). Novel peptide inhibitors for lactate dehydrogenase A (LDHA): a survey to inhibit LDHA activity via disruption of protein-protein interaction. Scientific reports, 9(1), 4686.

Feng, Y., Xiong, Y., Qiao, T., Li, X., Jia, L., & Han, Y. (2018). Lactate dehydrogenase A: A key player in carcinogenesis and potential target in cancer therapy. Cancer medicine, 7(12), 6124-6136.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Ldha ELISA Kit (CAT#: V2LY-0626-LY3275)

Mouse Recombinant LDHA protein, His Tag (CAT#: V2LY-0526-LY8610)

Rat Recombinant LDHA protein, His Tag (CAT#: V2LY-0526-LY9508)

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Rat Recombinant LDHA protein, His Tag (CAT#: V2LY-0526-LY9508)

Mouse Anti-LDHA Recombinant Antibody (20-179) (CAT#: CBMAB-L0935-YJ)

Mouse Ldha ELISA Kit (CAT#: V2LY-0626-LY3275)

Rabbit Anti-LDHA Recombinant Antibody (CBYJL-1341) (CAT#: CBMAB-L0937-YJ)

Mouse Anti-LDHA Recombinant Antibody (CBYJL-1339) (CAT#: CBMAB-L0934-YJ)

Mouse Anti-LDHA Recombinant Antibody (CBYJL-1349) (CAT#: CBMAB-L0951-YJ)

Mouse Anti-LDHA Recombinant Antibody (13F132) (CAT#: CBMAB-L0926-YJ)

Mouse Anti-LDHA Recombinant Antibody (2D11) (CAT#: CBMAB-L0940-YJ)

Mouse Anti-LDHA Recombinant Antibody (CBYJL-1335) (CAT#: CBMAB-L0929-YJ)

Isotype control

For research use only. Not intended for any clinical use.

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