Rat Ngb ELISA Kit (V2LY-0626-LY902)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0024 ng/mL
Detection Range
0.005-1 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
17.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Neuroglobin
Function
Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
Biological Process
Apoptotic processIEA:UniProtKB-KW
Oxygen transport1 PublicationNAS:UniProtKB
Cellular Location
Perikaryon
Cytoplasm
Mitochondrion
PTM
A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO.
Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity.

Xiang, Q., Zhou, D., Xiang, X., Le, X., Deng, C., Sun, R., ... & Xiang, T. (2023). Neuroglobin plays as tumor suppressor by disrupting the stability of GPR35 in colorectal cancer. Clinical Epigenetics, 15(1), 57.

Li, C. Y., Jiang, H. F., Li, L., Lai, X. J., Liu, Q. R., Yu, S. B., ... & Chen, X. Q. (2023). Neuroglobin Facilitates Neuronal Oxygenation through Tropic Migration under Hypoxia or Anemia in Rat: How Does the Brain Breathe?. Neuroscience Bulletin, 1-16.

Exertier, C., Montemiglio, L. C., Freda, I., Gugole, E., Parisi, G., Savino, C., & Vallone, B. (2022). Neuroglobin, clues to function and mechanism. Molecular Aspects of Medicine, 84, 101055.

Gøtzsche, C. R., Woldbye, D. P., Hundahl, C. A., & Hay‐Schmidt, A. (2022). Neuroglobin deficiency increases seizure susceptibility but does not affect basal behavior in mice. Journal of Neuroscience Research, 100(10), 1921-1932.

Ciccone, L., Nencetti, S., Socci, S., & Orlandini, E. (2021). Neuroglobin and neuroprotection: The role of natural and synthetic compounds in neuroglobin pharmacological induction. Neural regeneration research, 16(12), 2353.

De Simone, G., Sbardella, D., Oddone, F., Pesce, A., Coletta, M., & Ascenzi, P. (2021). Structural and (pseudo-) enzymatic properties of neuroglobin: its possible role in neuroprotection. Cells, 10(12), 3366.

Solar Fernandez, V., Fiocchetti, M., Cipolletti, M., Segatto, M., Cercola, P., Massari, A., ... & Marino, M. (2021). Neuroglobin: A new possible marker of estrogen-responsive breast cancer. Cells, 10(8), 1986.

Ding, C. Y., Kang, D. Z., Wang, Z. L., Lin, Y. X., Jiang, C. Z., Yu, L. H., ... & Gu, J. J. (2019). Serum Ngb (neuroglobin) is associated with brain metabolism and functional outcome of aneurysmal subarachnoid hemorrhage. Stroke, 50(7), 1887-1890.

Van Acker, Z. P., Luyckx, E., & Dewilde, S. (2019). Neuroglobin expression in the brain: a story of tissue homeostasis preservation. Molecular neurobiology, 56, 2101-2122.

Luyckx, E., Van Acker, Z. P., Ponsaerts, P., & Dewilde, S. (2019). Neuroglobin expression models as a tool to study its function. Oxidative medicine and cellular longevity, 2019.

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For research use only. Not intended for any clinical use.

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