Rat S100a1 ELISA Kit (V2LY-0626-LY2551)

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Tested Data
Request for COA
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Basic Information

Sensitivity
1.57 ng/mL
Detection Range
3-900 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
10.6 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
S100 Calcium Binding Protein A1
Function
Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600).
In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes (PubMed:23351007).
These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290).
Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca2+ cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca2+-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600).
Facilitates diastolic Ca2+ dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).
Biological Process
Biological Process intracellular signal transduction1 PublicationNAS:UniProtKB
Biological Process positive regulation of nitric-oxide synthase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process positive regulation of sprouting angiogenesisManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process regulation of heart contraction1 PublicationNAS:UniProtKB
Biological Process substantia nigra developmentManual Assertion Based On ExperimentHEP:UniProtKB
Cellular Location
Cytoplasm
Sarcoplasmic reticulum
Mitochondrion
PTM
Glutathionylated; glutathionylation increases affinity to calcium about 10-fold.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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