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Rabbit Anti-ACSS2 Recombinant Antibody (V2-179448) (CBMAB-A0759-YC)

Provided herein is a Rabbit monoclonal antibody against Human Acyl-CoA Synthetase Short Chain Family Member 2. The antibody can be used for immunoassay techniques, such as IP, WB.
See all ACSS2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Monkey, Mouse, Rat
Clone
V2-179448
Antibody Isotype
IgG
Application
IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to the sequence of human cytoplasmic acetyl-CoA synthetase (AceCS1)
Specificity
Human, Monkey, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in 10 mM sodium HEPES pH 7.5, 150 mM NaCl, 100 mg/mL BSA, 0.02% sodium azide, 50% glycerol
Buffer
HEPES, pH 7.5, 0.1mg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
acyl-CoA synthetase short-chain family member 2
Introduction
ACSS2 is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated
Entrez Gene ID
Human55902
Mouse60525
Rat311569
Monkey706703
UniProt ID
HumanQ9NR19
MouseQ9QXG4
RatG3V9U0
MonkeyF7EME7
Alternative Names
Acyl-CoA Synthetase Short Chain Family Member 2; Acetyl-Coenzyme A Synthetase 2 (ADP Forming); Acyl-Activating Enzyme; EC 6.2.1.1; ACECS; ACAS2; ACS; Acyl-CoA Synthetase Short-Chain Family Member 2; Acetyl-Coenzyme A Synthetase, Cytoplasmic;
Function
Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity (By similarity).
Biological Process
Acetate biosynthetic process
Acetyl-CoA biosynthetic process from acetate
Ethanol oxidation
Lipid biosynthetic process
Propionate biosynthetic process
Cellular Location
Cytosol
PTM
Reversibly acetylated at Lys-661 (By similarity). The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3 (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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