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Mouse Anti-ACSS2 Recombinant Antibody (V2-179457) (CBMAB-A0771-YC)

Provided herein is a Mouse monoclonal antibody against Human Acyl-CoA Synthetase Short Chain Family Member 2. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ACSS2 antibodies
Published Data
Mouse Anti-ACSS2 Recombinant Antibody (A-9) (CBMAB-A0771-YC) in WB
Representative Western blot images of lipogenesis marker ACSS2 in the liver, showing EEx-induced suppression of lipogenic proteins. ...View More
Cook, J. J., Wei, M., Segovia, B., Cosio-Lima, L., Simpson, J., Taylor, S., ... & Lee, Y. (2022). Endurance exercise-mediated metabolic reshuffle attenuates high-caloric diet-induced non-alcoholic fatty liver disease. Annals of Hepatology, 27(4), 100709.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179457
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Raised against amino acids 1-160 mapping at the N-terminus of ACSS2 of human origin
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
acyl-CoA synthetase short-chain family member 2
Introduction
ACSS2 is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated
Entrez Gene ID
55902
UniProt ID
Q9NR19
Alternative Names
Acyl-CoA Synthetase Short Chain Family Member 2; Acetyl-Coenzyme A Synthetase 2 (ADP Forming); Acyl-Activating Enzyme; EC 6.2.1.1; ACECS; ACAS2; ACS; Acyl-CoA Synthetase Short-Chain Family Member 2; Acetyl-Coenzyme A Synthetase, Cytoplasmic;
Function
Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity (By similarity).
Biological Process
Acetate biosynthetic process
Acetyl-CoA biosynthetic process from acetate
Ethanol oxidation
Lipid biosynthetic process
Propionate biosynthetic process
Cellular Location
Cytosol
PTM
Reversibly acetylated at Lys-661 (By similarity). The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3 (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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