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Mouse Anti-ALDH2 Recombinant Antibody (3D12) (CBMAB-AP263LY)


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Published Data
Mouse Anti-ALDH2 Recombinant Antibody (3D12) in Immunoblot
The expression level of glyoxalase 1 (GLO1; A), aldehyde dehydrogenase 2 (ALDH2; B), advanced glycation end products-receptor 1 (AGE-R1; C), and receptor for advanced glycation end products (RAGE; D) in PLA and SOL muscles following 4-wk voluntary exercise and/or MG treatment. Data are presented as means ± SD. n = 6/group. Individual data points are indicated on the bar graph. Representative immunoblots are shown. Statistical significance was analyzed using two-way ANOVA with exercise and MG treatment as main factors. *P < 0.05 with simple effects tests. ...View More
Egawa, T., Ogawa, T., Yokokawa, T., Kido, K., Goto, K., & Hayashi, T. (2022). Methylglyoxal reduces molecular responsiveness to 4 weeks of endurance exercise in mouse plantaris muscle. Journal of Applied Physiology, 132(2), 477-488.
Mouse Anti-ALDH2 Recombinant Antibody (3D12) in WB
Aortic protein expression of ALDH2 protein. Values are reported as and represent 5-6 mice. ...View More
Badran, M., Abuyassin, B., Golbidi, S., Ayas, N., & Laher, I. (2019). Alpha lipoic acid improves endothelial function and oxidative stress in mice exposed to chronic intermittent hypoxia. Oxidative medicine and cellular longevity, 2019.
Mouse Anti-ALDH2 Recombinant Antibody (3D12) in WB
Representative western blots of mitochondrial βIIPKC location in sham (white bars, n = 8), TAT-treated heart failure (HF-Ctr, gray bars, n = 5) and βIIV5-3-treated heart failure (HF-βIIV5-3, red bars, n = 7). ...View More
Ferreira, J. C., Campos, J. C., Qvit, N., Qi, X., Bozi, L. H., Bechara, L. R., ... & Mochly-Rosen, D. (2019). A selective inhibitor of mitofusin 1-βIIPKC association improves heart failure outcome in rats. Nature Communications, 10(1), 329.
Mouse Anti-ALDH2 Recombinant Antibody (3D12) in WB
Alterations in ALDH2 activity and the expression of ALDH2 mRNA and protein in H9c2 cardiac cells in different groups. Statistical results for (A) ALDH2 activity and (B) mRNA expression. (C) Representative western blot bands and (D) statistical analysis of ALDH2 expression. **P<0.01 vs. the control group; #P<0.05, ##P<0.01 vs. HG group. Data are expressed as the mean ± standard error of the mean (n=6). ...View More
Fang, T., Cao, R., Wang, W., Ye, H., Shen, L., Li, Z., ... & Gao, Q. (2018). Alterations in necroptosis during ALDH2‑mediated protection against high glucose‑induced H9c2 cardiac cell injury. Molecular Medicine Reports, 18(3), 2807-2815.
Mouse Anti-ALDH2 Recombinant Antibody (3D12) in Immunoblot
Representative immunoblots of ALDH1A1, ALDH1B1, ALDH2, and ALDH4A1 are shown with ERK2 as the loading control. Relative protein levels of all samples were determined by densitometry analysis normalized to ERK2 expression. Data are shown in box-whiskers graph (min to max value) (Normal, n = 8; steatosis, n = 4; NASH fatty, n = 11; NASH not fatty, n = 7). ...View More
Li, H., Toth, E., & Cherrington, N. J. (2018). Alcohol metabolism in the progression of human nonalcoholic steatohepatitis. Toxicological Sciences, 164(2), 428-438.
Mouse Anti-ALDH2 Recombinant Antibody (3D12) in WB
Representative western blotting results are shown. ALDH2, aldehyde dehydrogenase 2; CLP, cecal ligation and puncture; CYA, cyanamide. ...View More
Hu, J. F., Wang, H. X., Li, H. H., Hu, J., Yu, Y., & Gao, Q. (2017). Inhibition of ALDH2 expression aggravates renal injury in a rat sepsis syndrome model. Experimental and therapeutic medicine, 14(3), 2249-2254.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
3D12
Antibody Isotype
IgG2a, κ
Application
IP, ELISA, WB

Basic Information

Immunogen
Recombinant ALDH2 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Aldehyde Dehydrogenase 2 Family (Mitochondrial)
Introduction
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Nov 2016]
Entrez Gene ID
217
UniProt ID
P05091
Alternative Names
Aldehyde Dehydrogenase 2 Family (Mitochondrial); ALDH Class 2; EC 1.2.1.3; ALDH-E2; ALDHI; ALDM;
Biological Process
Alcohol metabolic process Source: ProtInc
Carbohydrate metabolic process Source: ProtInc
Ethanol catabolic process Source: UniProtKB-UniPathway
Ethanol oxidation Source: Reactome
Cellular Location
Mitochondrion matrix
More Infomation

Zhu, Z. Y., Liu, Y. D., Gong, Y., Jin, W., Topchiy, E., Turdi, S., ... & Qin, X. (2021). Mitochondrial aldehyde dehydrogenase (ALDH2) rescues cardiac contractile dysfunction in an APP/PS1 murine model of Alzheimer’s disease via inhibition of ACSL4-dependent ferroptosis. Acta Pharmacologica Sinica, 1-11.

Chen, L., Wu, Y. T., Gu, X. Y., Xie, L. P., Fan, H. J., Tan, Z. B., ... & Sun, X. M. (2021). Magnolol, a natural aldehyde dehydrogenase-2 agonist, inhibits the proliferation and collagen synthesis of cardiac fibroblasts. Bioorganic & Medicinal Chemistry Letters, 43, 128045.

Suo, C., Yang, Y., Yuan, Z., Zhang, T., Yang, X., Qing, T., ... & Ye, W. (2019). Alcohol intake interacts with functional genetic polymorphisms of aldehyde dehydrogenase (ALDH2) and alcohol dehydrogenase (ADH) to increase esophageal squamous cell cancer risk. Journal of Thoracic Oncology, 14(4), 712-725.

Zhao, Y., Wang, B., Zhang, J., He, D., Zhang, Q., Pan, C., ... & Chen, Y. (2019). ALDH2 (Aldehyde Dehydrogenase 2) protects against hypoxia-induced pulmonary hypertension. Arteriosclerosis, thrombosis, and vascular biology, 39(11), 2303-2319.

Kimura, M., Yokoyama, A., & Higuchi, S. (2019). Aldehyde dehydrogenase-2 as a therapeutic target. Expert opinion on therapeutic targets, 23(11), 955-966.

Wang, Y., & He, W. (2018). Endogenous Mitochondrial Aldehyde Dehydrogenase-2 as an Antioxidant in Liver. In The Liver (pp. 247-259). Academic Press.

Miura, T., Nishinaka, T., Terada, T., & Yonezawa, K. (2017). Vasodilatory effect of nitroglycerin in Japanese subjects with different aldehyde dehydrogenase 2 (ALDH2) genotypes. Chemico-biological interactions, 276, 40-45.

Buchman, C. D., & Hurley, T. D. (2017). Inhibition of the aldehyde dehydrogenase 1/2 family by psoralen and coumarin derivatives. Journal of medicinal chemistry, 60(6), 2439-2455.

Nene, A., Chen, C. H., Disatnik, M. H., Cruz, L., & Mochly-Rosen, D. (2017). Aldehyde dehydrogenase 2 activation and coevolution of its εPKC-mediated phosphorylation sites. Journal of biomedical science, 24(1), 1-12.

Angenendt, L., Bormann, E., Mikesch, J. H., Herold, T., Metzeler, K. H., Bohlander, S. K., ... & Schliemann, C. (2017). Aldehyde Dehydrogenase 2 (ALDH2) Expression Predicts a Poor Prognosis in Acute Myeloid Leukemia. Blood, 130, 1386.

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For research use only. Not intended for any clinical use.

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