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Mouse Anti-AMPH Monoclonal Antibody (13) (CBMAB-0940-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
13
Antibody Isotype
IgG1, κ
Application
IF, IP, WB

Basic Information

Immunogen
N-terminus of Amphiphysin I of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Amphiphysin
Introduction
Amphiphysin is a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein.
Entrez Gene ID
Human273
Mouse218038
Rat60668
Cattle614722
Dog475877
Hamster100767913
Monkey704559
UniProt ID
HumanP49418
MouseQ7TQF7
RatO08838
CattleA5D783
DogF1P6S5
HamsterA0A1U7QS39
MonkeyA0A2K5X711
RabbitG1SH47
Alternative Names
AMPH1
Function
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Biological Process
Chemical synaptic transmission Source: ProtInc
Endocytosis Source: ProtInc
Membrane organization Source: Reactome
Synaptic vesicle endocytosis Source: GO_Central
Cellular Location
Cytoskeleton; Synaptic vesicle membrane
More Infomation

Kapoor, T., Dubey, P., Shirolikar, S., & Ray, K. (2021). An actomyosin clamp assembled by the Amphiphysin-Rho1-Dia/DAAM-Rok pathway reinforces somatic cell membrane folded around spermatid heads. Cell Reports, 34(13), 108918.

Zhang, X., Zou, L., Meng, L., Xiong, M., Pan, L., Chen, G., ... & Zhang, Z. (2021). Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. Elife, 10, e65301.

Kapoor, T., Dubey, P., Shirolikar, S., & Ray, K. (2020). Nanoscale indentation of plasma membrane establishes a contractile actomyosin scaffold through selective activation of the Amphiphysin-Rho1-Dia/DAAM and Rok pathway. DAAM and Rok Pathway.

Zhang, H., Liu, Y., Xu, K., Mao, K., Han, W., Xu, F., ... & Sun, Y. (2019). AMPH-1 as a critical tumor suppressor that inhibits osteosarcoma progression. Cancer management and research, 11, 9913.

Lionello, V. M., Nicot, A. S., Sartori, M., Kretz, C., Kessler, P., Buono, S., ... & Cowling, B. S. (2019). Amphiphysin 2 modulation rescues myotubular myopathy and prevents focal adhesion defects in mice. Science translational medicine, 11(484).

Yang, H., Wan, Z., Huang, C., Yin, H., & Song, D. (2019). AMPH-1 is a tumor suppressor of lung cancer by inhibiting Ras-Raf-MEK-ERK signal pathway. Lasers in medical science, 34(3), 473-478.

Jiang, L., Ge, W., & Geng, J. (2019). miR-425 regulates cell proliferation, migration and apoptosis by targeting AMPH-1 in non-small-cell lung cancer. Pathology-Research and Practice, 215(12), 152705.

Chen, Y., Liu, J., Li, L., Xia, H., Lin, Z., & Zhong, T. (2018). AMPH-1 is critical for breast cancer progression. Journal of Cancer, 9(12), 2175.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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