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Mouse Anti-AMPH Recombinant Antibody (CBYC-A570) (CBMAB-A2581-YC)

Provided herein is a Mouse monoclonal antibody against Human Amphiphysin. The antibody can be used for immunoassay techniques, such as WB.
See all AMPH antibodies
Published Data
Mouse Anti-AMPH Recombinant Antibody (CBYC-A570) in WB
Cleavage of mutant Amph I by AEP. Amph I cleavage was analyzed by Western blot after glutathione S-transferase (GST)-Amph I wild-type or N278A mutant was incubated with recombinant AEP. ...View More
Zhang, X., Zou, L., Meng, L., Xiong, M., Pan, L., Chen, G., ... & Zhang, Z. (2021). Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. Elife, 10, e65301.
Mouse Anti-AMPH Recombinant Antibody (CBYC-A570) in IHC
Immunohistochemistry (IHC) of Amph I N278 fragments in brain sections from 8-month-old Tau P301S mice and wild-type mice (mean ± s.e.m.; Student's t-test, n = 4 mice per group; ***p<0.001). Scale bar, 20 μm. ...View More
Zhang, X., Zou, L., Meng, L., Xiong, M., Pan, L., Chen, G., ... & Zhang, Z. (2021). Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. Elife, 10, e65301.
Mouse Anti-AMPH Recombinant Antibody (CBYC-A570) in IF
Transferrin uptake assay. Confocal laser scanning micrographs showing the uptake of transferrin by COS-7 cells expressing EGFP, EGFP-Amph I FL, EGFP-Amph I (1-278), EGFP-Amph I (279-695), and EGFP-active AEP (26-323), respectively. Top: Transferrin uptake in transfected cells; middle, EGFP expression in COS-7 cells; bottom, the merge of EGFP and transferrin uptake. The arrows indicate transferrin uptaken by transfected cells. Scale bar, 20 μm. ...View More
Zhang, X., Zou, L., Meng, L., Xiong, M., Pan, L., Chen, G., ... & Zhang, Z. (2021). Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. Elife, 10, e65301.
Mouse Anti-AMPH Recombinant Antibody (CBYC-A570) in IF
Colocalization of SYNJ1 and its fragments (Green) with synaptic proteins including amphiphysin I, endophilin A1, and dynamin I (Red). Scale bar, 10 μm. ...View More
Zou, L., Zhang, X., Xiong, M., Meng, L., Tian, Y., Pan, L., ... & Zhang, Z. (2021). Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease. Neurobiology of Disease, 154, 105326.
Mouse Anti-AMPH Recombinant Antibody (CBYC-A570) in Immunoblot
Immunoblot analysis of APP, BACE1, GFAP and Amph1 levels in 5XFAD and 5XFADBin1+/- mice. ...View More
Andrew, R. J., De Rossi, P., Nguyen, P., Kowalski, H. R., Recupero, A. J., Guerbette, T., ... & Thinakaran, G. (2019). Reduction of the expression of the late-onset Alzheimer's disease (AD) risk-factor BIN1 does not affect amyloid pathology in an AD mouse model. Journal of Biological Chemistry, 294(12), 4477-4487.
Mouse Anti-AMPH Recombinant Antibody (CBYC-A570) in WB
Alternatively spliced amphI iso2 is phosphorylated in nerve terminals. GST--adaptin appendage domain and GST-endophilin I SH3 domain were used in a double pulldown experiment to isolate 32P-labeled amphI. Western blotting (WB) using an anti-phospho-Ser-262 antibody detected two major bands corresponding to amphIlow and a lower band at 110 kDa (lanes 5 and 6). Coomassie Blue protein stain (lanes 1 and 2) and autoradiography (Autorad; lanes 3 and 4) indicated a low level of protein staining and 32P incorporation in the amphI iso2. CON, control; DEP, depolarization. ...View More
Craft, G. E., Graham, M. E., Bache, N., Larsen, M. R., & Robinson, P. J. (2008). The in vivo phosphorylation sites in multiple isoforms of amphiphysin I from rat brain nerve terminals. Molecular & Cellular Proteomics, 7(6), 1146-1161.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-A570
Antibody Isotype
IgG2b, κ
Application
WB, ICC, IHC-P, IP

Basic Information

Immunogen
N-terminus of Amphiphysin I of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Amphiphysin
Introduction
AMPH is a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene resu
Entrez Gene ID
Human273
Mouse218038
UniProt ID
HumanP49418
MouseQ7TQF7
Alternative Names
Amphiphysin; Amphiphysin (Stiff-Mann Syndrome With Breast Cancer 128kD Autoantigen); AMPH1; Amphiphysin (Stiff-Man Syndrome With Breast Cancer 128kDa Autoantigen); Amphiphysin I;
Function
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Biological Process
Chemical synaptic transmission Source: ProtInc
Endocytosis Source: ProtInc
Membrane organization Source: Reactome
Synaptic vesicle endocytosis Source: GO_Central
Cellular Location
Cytoskeleton; Synaptic vesicle membrane

Kapoor, T., Dubey, P., Shirolikar, S., & Ray, K. (2021). An actomyosin clamp assembled by the Amphiphysin-Rho1-Dia/DAAM-Rok pathway reinforces somatic cell membrane folded around spermatid heads. Cell Reports, 34(13), 108918.

Zhang, X., Zou, L., Meng, L., Xiong, M., Pan, L., Chen, G., ... & Zhang, Z. (2021). Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. Elife, 10, e65301.

Kapoor, T., Dubey, P., Shirolikar, S., & Ray, K. (2020). Nanoscale indentation of plasma membrane establishes a contractile actomyosin scaffold through selective activation of the Amphiphysin-Rho1-Dia/DAAM and Rok pathway. DAAM and Rok Pathway.

Zhang, H., Liu, Y., Xu, K., Mao, K., Han, W., Xu, F., ... & Sun, Y. (2019). AMPH-1 as a critical tumor suppressor that inhibits osteosarcoma progression. Cancer management and research, 11, 9913.

Lionello, V. M., Nicot, A. S., Sartori, M., Kretz, C., Kessler, P., Buono, S., ... & Cowling, B. S. (2019). Amphiphysin 2 modulation rescues myotubular myopathy and prevents focal adhesion defects in mice. Science translational medicine, 11(484).

Yang, H., Wan, Z., Huang, C., Yin, H., & Song, D. (2019). AMPH-1 is a tumor suppressor of lung cancer by inhibiting Ras-Raf-MEK-ERK signal pathway. Lasers in medical science, 34(3), 473-478.

Jiang, L., Ge, W., & Geng, J. (2019). miR-425 regulates cell proliferation, migration and apoptosis by targeting AMPH-1 in non-small-cell lung cancer. Pathology-Research and Practice, 215(12), 152705.

Chen, Y., Liu, J., Li, L., Xia, H., Lin, Z., & Zhong, T. (2018). AMPH-1 is critical for breast cancer progression. Journal of Cancer, 9(12), 2175.

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For research use only. Not intended for any clinical use.

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