Sign in or Register   Sign in or Register
  |  

Mouse Anti-AMPH Recombinant Antibody (CBLNA-114) (CBMAB-1074-CN)

This product is a mouse antibody that recognizes AMPH of human. The antibody CBLNA-114 can be used for immunoassay techniques such as: WB, ICC, IHC-P, IP.
See all AMPH antibodies
Published Data
Mouse Anti-AMPH Recombinant Antibody (CBLNA-114) in IF
Uptake of Alexa Fluor 594-transferrin was visualized in bronchioles of siRNA-treated mouse lung expressing GFP (control) and amphiphysin (AMPH-WT or AMPH-V392G). Scale bar, 20 m. siPIXm1, mouse specific siRNA for silencing PIX. ...View More
Shin, E. Y., Park, J. H., You, S. T., Lee, C. S., Won, S. Y., Park, J. J., ... & Kim, E. G. (2020). Integrin-mediated adhesions in regulation of cellular senescence. Science Advances, 6(19), eaay3909.
Mouse Anti-AMPH Recombinant Antibody (CBLNA-114) in IHC
SA--Gal and immunohistochemical staining for senescence markers were performed in the bronchioles of siRNA-treated mice expressing GFP (control) and amphiphysin (AMPH-WT or AMPH-V392G). Red arrows indicate positive staining. Scale bars, 10 m. ...View More
Shin, E. Y., Park, J. H., You, S. T., Lee, C. S., Won, S. Y., Park, J. J., ... & Kim, E. G. (2020). Integrin-mediated adhesions in regulation of cellular senescence. Science Advances, 6(19), eaay3909.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBLNA-114
Antibody Isotype
IgG2b
Application
WB, ICC, IHC-P, IP, ELISA

Basic Information

Immunogen
Amino acids 2-20 at the N-terminus of Amphiphysin of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
Localized in aa.1-347

Target

Full Name
Amphiphysin
Introduction
This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11. The protein may participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. The protein may control the properties of the membrane associated cytoskeleton.
Entrez Gene ID
Human273
Mouse218038
Rat60668
UniProt ID
HumanP49418
MouseQ7TQF7
RatO08838
Alternative Names
AMPH1
Function
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Biological Process
Chemical synaptic transmission Source: ProtInc
Endocytosis Source: ProtInc
Membrane organization Source: Reactome
Synaptic vesicle endocytosis Source: GO_Central
Cellular Location
Cytoskeleton; Synaptic vesicle membrane

Kapoor, T., Dubey, P., Shirolikar, S., & Ray, K. (2021). An actomyosin clamp assembled by the Amphiphysin-Rho1-Dia/DAAM-Rok pathway reinforces somatic cell membrane folded around spermatid heads. Cell Reports, 34(13), 108918.

Zhang, X., Zou, L., Meng, L., Xiong, M., Pan, L., Chen, G., ... & Zhang, Z. (2021). Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. Elife, 10, e65301.

Kapoor, T., Dubey, P., Shirolikar, S., & Ray, K. (2020). Nanoscale indentation of plasma membrane establishes a contractile actomyosin scaffold through selective activation of the Amphiphysin-Rho1-Dia/DAAM and Rok pathway. DAAM and Rok Pathway.

Zhang, H., Liu, Y., Xu, K., Mao, K., Han, W., Xu, F., ... & Sun, Y. (2019). AMPH-1 as a critical tumor suppressor that inhibits osteosarcoma progression. Cancer management and research, 11, 9913.

Lionello, V. M., Nicot, A. S., Sartori, M., Kretz, C., Kessler, P., Buono, S., ... & Cowling, B. S. (2019). Amphiphysin 2 modulation rescues myotubular myopathy and prevents focal adhesion defects in mice. Science translational medicine, 11(484).

Yang, H., Wan, Z., Huang, C., Yin, H., & Song, D. (2019). AMPH-1 is a tumor suppressor of lung cancer by inhibiting Ras-Raf-MEK-ERK signal pathway. Lasers in medical science, 34(3), 473-478.

Jiang, L., Ge, W., & Geng, J. (2019). miR-425 regulates cell proliferation, migration and apoptosis by targeting AMPH-1 in non-small-cell lung cancer. Pathology-Research and Practice, 215(12), 152705.

Chen, Y., Liu, J., Li, L., Xia, H., Lin, Z., & Zhong, T. (2018). AMPH-1 is critical for breast cancer progression. Journal of Cancer, 9(12), 2175.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-AMPH Recombinant Antibody (CBLNA-114)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare