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Mouse Anti-AMTN Recombinant Antibody (1C11) (CBMAB-A2593-YC)

Provided herein is a Mouse monoclonal antibody against Human Amelotin. The antibody can be used for immunoassay techniques, such as IHC, WB.
See all AMTN antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1C11
Antibody Isotype
IgG1
Application
IHC, WB

Basic Information

Immunogen
Full length human recombinant protein of human AMTN (NP_997722) produced in E. coli
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500
IHC1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Amelotin
Introduction
The mineralized portions of teeth, the dentin and enamel, are formed by mesenchyme-derived odontoblasts and epithelium-derived ameloblasts, respectively. As ameloblasts differentiate, they deposit specific proteins necessary for enamel formation, includin
Entrez Gene ID
401138
UniProt ID
Q6UX39
Alternative Names
Amelotin; RSTI689; UNQ689; AI3B;
Function
Is a promoter of calcium phosphate mineralization, playing a critical role in the formation of the compact, mineralized, aprismatic enamel surface layer during the maturation stage of amelogenesis.
Biological Process
Biomineral tissue development Source: UniProtKB-KW
Cell adhesion Source: UniProtKB-KW
Cellular protein metabolic process Source: Reactome
Odontogenesis of dentin-containing tooth Source: HGNC-UCL
Positive regulation of biomineral tissue development Source: UniProtKB
Positive regulation of enamel mineralization Source: GO_Central
Post-translational protein modification Source: Reactome
Cellular Location
Secreted
Involvement in disease
Amelogenesis imperfecta 3B (AI3B): An autosomal dominant form of amelogenesis imperfecta, a defect of enamel formation. AI3B is characterized by hypomineralized enamel that has reduced tickness and exhibits structural defects.
PTM
Phosphorylated by FAM20C in vitro.
O-glycosylated.

Athanasiadou, D., Danesi, A. L., Umbrio, L., Holcroft, J., Ganss, B., & Carneiro, K. M. (2021). Hydroxyapatite Growth on Amelogenin‐Amelotin Recombinamers. ChemNanoMat.

Rajapakse, D., Peterson, K., Mishra, S., Fan, J., Lerner, J., Campos, M., & Wistow, G. (2020). Amelotin is expressed in retinal pigment epithelium and localizes to hydroxyapatite deposits in dry age-related macular degeneration. Translational Research, 219, 45-62.

Zhang, J., Wang, L., Zhang, W., & Putnis, C. V. (2020). Phosphorylated/Nonphosphorylated Motifs in Amelotin Turn Off/On the Acidic Amorphous Calcium Phosphate-to-Apatite Phase Transformation. Langmuir, 36(8), 2102-2109.

Nakayama, Y., Tsuruya, Y., Noda, K., Yamazaki‐Takai, M., Iwai, Y., Ganss, B., & Ogata, Y. (2019). Negative feedback by SNAI2 regulates TGFβ1‐induced amelotin gene transcription in epithelial–mesenchymal transition.

Yu, M., Wang, L., Zhang, W., & Ganss, B. (2019). An evolutionarily conserved subdomain in amelotin promotes amorphous calcium phosphate-to-hydroxyapatite phase transition. Crystal Growth & Design, 19(4), 2104-2113.

Yu, M., Wang, L., Zhang, W., & Ganss, B. (2018). A highly conserved motif within the amelotin protein controls the surface growth of brushite. Crystal Growth & Design, 18(4), 2502-2509.

Yamazaki, M., Mezawa, M., Noda, K., Iwai, Y., Matsui, S., Takai, H., ... & Ogata, Y. (2018). Transcriptional regulation of human amelotin gene by interleukin‐1β. FEBS Open bio, 8(6), 974-985.

Yamazaki, M., Iwai, Y., Noda, K., Matsui, S., Kato, A., Takai, H., ... & Ogata, Y. (2018). Tumor necrosis factor-α stimulates human amelotin gene transcription in gingival epithelial cells. Inflammation Research, 67(4), 351-361.

Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C., ... & Gao, Y. (2018). Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis. Molecular medicine reports, 17(4), 6068-6076.

Nakayama, Y., Matsui, S., Noda, K., Yamazaki, M., Iwai, Y., Ganss, B., & Ogata, Y. (2018). TGFβ1-induced Amelotin gene expression is downregulated by Bax expression in mouse gingival epithelial cells. Journal of oral science, 17-0271.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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