Human AMTN ELISA Kit (V2LY-0626-LY4339)

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Tested Data
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Basic Information

Sensitivity
0.026 ng/mL
Detection Range
0.05-10 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
21.6 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Amelotin
Function
Is a promoter of calcium phosphate mineralization, playing a critical role in the formation of the compact, mineralized, aprismatic enamel surface layer during the maturation stage of amelogenesis.
Biological Process
Biomineral tissue development Source: UniProtKB-KW
Cell adhesion Source: UniProtKB-KW
Cellular protein metabolic process Source: Reactome
Odontogenesis of dentin-containing tooth Source: HGNC-UCL
Positive regulation of biomineral tissue development Source: UniProtKB
Positive regulation of enamel mineralization Source: GO_Central
Post-translational protein modification Source: Reactome
Cellular Location
Secreted
Involvement in disease
Amelogenesis imperfecta 3B (AI3B): An autosomal dominant form of amelogenesis imperfecta, a defect of enamel formation. AI3B is characterized by hypomineralized enamel that has reduced tickness and exhibits structural defects.
PTM
Phosphorylated by FAM20C in vitro.
O-glycosylated.

Athanasiadou, D., Danesi, A. L., Umbrio, L., Holcroft, J., Ganss, B., & Carneiro, K. M. (2021). Hydroxyapatite Growth on Amelogenin‐Amelotin Recombinamers. ChemNanoMat.

Rajapakse, D., Peterson, K., Mishra, S., Fan, J., Lerner, J., Campos, M., & Wistow, G. (2020). Amelotin is expressed in retinal pigment epithelium and localizes to hydroxyapatite deposits in dry age-related macular degeneration. Translational Research, 219, 45-62.

Zhang, J., Wang, L., Zhang, W., & Putnis, C. V. (2020). Phosphorylated/Nonphosphorylated Motifs in Amelotin Turn Off/On the Acidic Amorphous Calcium Phosphate-to-Apatite Phase Transformation. Langmuir, 36(8), 2102-2109.

Nakayama, Y., Tsuruya, Y., Noda, K., Yamazaki‐Takai, M., Iwai, Y., Ganss, B., & Ogata, Y. (2019). Negative feedback by SNAI2 regulates TGFβ1‐induced amelotin gene transcription in epithelial–mesenchymal transition.

Yu, M., Wang, L., Zhang, W., & Ganss, B. (2019). An evolutionarily conserved subdomain in amelotin promotes amorphous calcium phosphate-to-hydroxyapatite phase transition. Crystal Growth & Design, 19(4), 2104-2113.

Yu, M., Wang, L., Zhang, W., & Ganss, B. (2018). A highly conserved motif within the amelotin protein controls the surface growth of brushite. Crystal Growth & Design, 18(4), 2502-2509.

Yamazaki, M., Mezawa, M., Noda, K., Iwai, Y., Matsui, S., Takai, H., ... & Ogata, Y. (2018). Transcriptional regulation of human amelotin gene by interleukin‐1β. FEBS Open bio, 8(6), 974-985.

Yamazaki, M., Iwai, Y., Noda, K., Matsui, S., Kato, A., Takai, H., ... & Ogata, Y. (2018). Tumor necrosis factor-α stimulates human amelotin gene transcription in gingival epithelial cells. Inflammation Research, 67(4), 351-361.

Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C., ... & Gao, Y. (2018). Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis. Molecular medicine reports, 17(4), 6068-6076.

Nakayama, Y., Matsui, S., Noda, K., Yamazaki, M., Iwai, Y., Ganss, B., & Ogata, Y. (2018). TGFβ1-induced Amelotin gene expression is downregulated by Bax expression in mouse gingival epithelial cells. Journal of oral science, 17-0271.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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