Human Recombinant AMTN protein, Fc Tag (V2LY-0526-LY2069)

Name: Human Recombinant AMTN protein, Fc Tag
SKU: V2LY-0526-LY2069
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

Fc Tag

Protein Construction

This product is Human Recombinant AMTN protein, Fc Tag consist of Amino Acid: 1-209 and predicts a molecular mass of 46.2 kDa.

Molecule Mass

46.2 kDa

Sequence

Amino Acid: 1-209

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>78% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Amelotin

Function

Is a promoter of calcium phosphate mineralization, playing a critical role in the formation of the compact, mineralized, aprismatic enamel surface layer during the maturation stage of amelogenesis.

Biological Process

Biomineral tissue development Source: UniProtKB-KW
Cell adhesion Source: UniProtKB-KW
Cellular protein metabolic process Source: Reactome
Odontogenesis of dentin-containing tooth Source: HGNC-UCL
Positive regulation of biomineral tissue development Source: UniProtKB
Positive regulation of enamel mineralization Source: GO_Central
Post-translational protein modification Source: Reactome

Cellular Location

Secreted

Involvement in disease

Amelogenesis imperfecta 3B (AI3B): An autosomal dominant form of amelogenesis imperfecta, a defect of enamel formation. AI3B is characterized by hypomineralized enamel that has reduced tickness and exhibits structural defects.

PTM

Phosphorylated by FAM20C in vitro.
O-glycosylated.

Athanasiadou, D., Danesi, A. L., Umbrio, L., Holcroft, J., Ganss, B., & Carneiro, K. M. (2021). Hydroxyapatite Growth on Amelogenin‐Amelotin Recombinamers. ChemNanoMat.

Rajapakse, D., Peterson, K., Mishra, S., Fan, J., Lerner, J., Campos, M., & Wistow, G. (2020). Amelotin is expressed in retinal pigment epithelium and localizes to hydroxyapatite deposits in dry age-related macular degeneration. Translational Research, 219, 45-62.

Zhang, J., Wang, L., Zhang, W., & Putnis, C. V. (2020). Phosphorylated/Nonphosphorylated Motifs in Amelotin Turn Off/On the Acidic Amorphous Calcium Phosphate-to-Apatite Phase Transformation. Langmuir, 36(8), 2102-2109.

Nakayama, Y., Tsuruya, Y., Noda, K., Yamazaki‐Takai, M., Iwai, Y., Ganss, B., & Ogata, Y. (2019). Negative feedback by SNAI2 regulates TGFβ1‐induced amelotin gene transcription in epithelial–mesenchymal transition.

Yu, M., Wang, L., Zhang, W., & Ganss, B. (2019). An evolutionarily conserved subdomain in amelotin promotes amorphous calcium phosphate-to-hydroxyapatite phase transition. Crystal Growth & Design, 19(4), 2104-2113.

Yu, M., Wang, L., Zhang, W., & Ganss, B. (2018). A highly conserved motif within the amelotin protein controls the surface growth of brushite. Crystal Growth & Design, 18(4), 2502-2509.

Yamazaki, M., Mezawa, M., Noda, K., Iwai, Y., Matsui, S., Takai, H., ... & Ogata, Y. (2018). Transcriptional regulation of human amelotin gene by interleukin‐1β. FEBS Open bio, 8(6), 974-985.

Yamazaki, M., Iwai, Y., Noda, K., Matsui, S., Kato, A., Takai, H., ... & Ogata, Y. (2018). Tumor necrosis factor-α stimulates human amelotin gene transcription in gingival epithelial cells. Inflammation Research, 67(4), 351-361.

Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C., ... & Gao, Y. (2018). Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis. Molecular medicine reports, 17(4), 6068-6076.

Nakayama, Y., Matsui, S., Noda, K., Yamazaki, M., Iwai, Y., Ganss, B., & Ogata, Y. (2018). TGFβ1-induced Amelotin gene expression is downregulated by Bax expression in mouse gingival epithelial cells. Journal of oral science, 17-0271.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human AMTN ELISA Kit (CAT#: V2LY-0626-LY4339)

Related Products

Mouse Anti-AMTN Recombinant Antibody (2D9) (CAT#: CBMAB-A2597-YC)

Mouse Anti-AMTN Recombinant Antibody (1C11) (CAT#: CBMAB-A2593-YC)

Human AMTN ELISA Kit (CAT#: V2LY-0626-LY4339)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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