Mouse Anti-AMTN Recombinant Antibody (2D9) (CBMAB-A2597-YC)

Name: Mouse Anti-AMTN Recombinant Antibody (2D9)
SKU: CBMAB-A2597-YC
Rating: 5 (1 reviews)

See all AMTN antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2D9

Application

WB, IHC

Immunogen

Full length human recombinant protein of human AMTN (NP_997722) produced in E. coli

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
IHC	1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Amelotin

Introduction

The mineralized portions of teeth, the dentin and enamel, are formed by mesenchyme-derived odontoblasts and epithelium-derived ameloblasts, respectively. As ameloblasts differentiate, they deposit specific proteins necessary for enamel formation, includin

Entrez Gene ID

401138

UniProt ID

Q6UX39

Alternative Names

Amelotin; RSTI689; UNQ689; AI3B;

Function

Is a promoter of calcium phosphate mineralization, playing a critical role in the formation of the compact, mineralized, aprismatic enamel surface layer during the maturation stage of amelogenesis.

Biological Process

Biomineral tissue development Source: UniProtKB-KW
Cell adhesion Source: UniProtKB-KW
Cellular protein metabolic process Source: Reactome
Odontogenesis of dentin-containing tooth Source: HGNC-UCL
Positive regulation of biomineral tissue development Source: UniProtKB
Positive regulation of enamel mineralization Source: GO_Central
Post-translational protein modification Source: Reactome

Cellular Location

Secreted

Involvement in disease

Amelogenesis imperfecta 3B (AI3B): An autosomal dominant form of amelogenesis imperfecta, a defect of enamel formation. AI3B is characterized by hypomineralized enamel that has reduced tickness and exhibits structural defects.

PTM

Phosphorylated by FAM20C in vitro.
O-glycosylated.

Athanasiadou, D., Danesi, A. L., Umbrio, L., Holcroft, J., Ganss, B., & Carneiro, K. M. (2021). Hydroxyapatite Growth on Amelogenin‐Amelotin Recombinamers. ChemNanoMat.

Rajapakse, D., Peterson, K., Mishra, S., Fan, J., Lerner, J., Campos, M., & Wistow, G. (2020). Amelotin is expressed in retinal pigment epithelium and localizes to hydroxyapatite deposits in dry age-related macular degeneration. Translational Research, 219, 45-62.

Zhang, J., Wang, L., Zhang, W., & Putnis, C. V. (2020). Phosphorylated/Nonphosphorylated Motifs in Amelotin Turn Off/On the Acidic Amorphous Calcium Phosphate-to-Apatite Phase Transformation. Langmuir, 36(8), 2102-2109.

Nakayama, Y., Tsuruya, Y., Noda, K., Yamazaki‐Takai, M., Iwai, Y., Ganss, B., & Ogata, Y. (2019). Negative feedback by SNAI2 regulates TGFβ1‐induced amelotin gene transcription in epithelial–mesenchymal transition.

Yu, M., Wang, L., Zhang, W., & Ganss, B. (2019). An evolutionarily conserved subdomain in amelotin promotes amorphous calcium phosphate-to-hydroxyapatite phase transition. Crystal Growth & Design, 19(4), 2104-2113.

Yu, M., Wang, L., Zhang, W., & Ganss, B. (2018). A highly conserved motif within the amelotin protein controls the surface growth of brushite. Crystal Growth & Design, 18(4), 2502-2509.

Yamazaki, M., Mezawa, M., Noda, K., Iwai, Y., Matsui, S., Takai, H., ... & Ogata, Y. (2018). Transcriptional regulation of human amelotin gene by interleukin‐1β. FEBS Open bio, 8(6), 974-985.

Yamazaki, M., Iwai, Y., Noda, K., Matsui, S., Kato, A., Takai, H., ... & Ogata, Y. (2018). Tumor necrosis factor-α stimulates human amelotin gene transcription in gingival epithelial cells. Inflammation Research, 67(4), 351-361.

Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C., ... & Gao, Y. (2018). Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis. Molecular medicine reports, 17(4), 6068-6076.

Nakayama, Y., Matsui, S., Noda, K., Yamazaki, M., Iwai, Y., Ganss, B., & Ogata, Y. (2018). TGFβ1-induced Amelotin gene expression is downregulated by Bax expression in mouse gingival epithelial cells. Journal of oral science, 17-0271.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-AMTN Recombinant Antibody (2D9)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-AMTN Recombinant Antibody (1C11) (CAT#: CBMAB-A2593-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry