Mouse Anti-ASF1B Recombinant Antibody (CBT3032) (V2LY-0625-LY715)

Name: Mouse Anti-ASF1B Recombinant Antibody (CBT3032)
SKU: V2LY-0625-LY715
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ASF1B Recombinant Antibody (CBT3032) (V2LY-0625-LY715) in ELISA

Mouse Anti-ASF1B Recombinant Antibody (CBT3032) (V2LY-0625-LY715) in WB

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3032

Application

Immunogen

Purified recombinant fragment of human ASF1B (AA: 1-202) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Anti-Silencing Function 1B Histone Chaperone

Entrez Gene ID

55723

UniProt ID

Q9NVP2

Function

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.

Biological Process

Blastocyst hatching Source: Ensembl
Cell differentiation Source: UniProtKB-KW
DNA replication-dependent nucleosome assembly Source: UniProtKB
DNA replication-independent nucleosome assembly Source: UniProtKB
Spermatogenesis Source: UniProtKB-KW

Cellular Location

Nucleus

PTM

Phosphorylated by TLK1 and TLK2.

Jiangqiao, Z., Tao, Q., Zhongbao, C., Xiaoxiong, M., Long, Z., Jilin, Z., & Tianyu, W. (2019). Anti-silencing function 1B histone chaperone promotes cell proliferation and migration via activation of the AKT pathway in clear cell renal cell carcinoma. Biochemical and biophysical research communications, 511(1), 165-172.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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