Sign in or Register   Sign in or Register
  |  

Rabbit Anti-ASF1B Recombinant Antibody (EG272) (CBMAB-EN300-LY)

The product is antibody recognizes ASF1B . The antibody EG272 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:1000.
See all ASF1B antibodies
Published Data
Rabbit Anti-ASF1B Recombinant Antibody (EG272) CBMAB-EN300-LY in IF
Analysis of Asf1b and MCM2 localization by confocal microscopy. HeLa S3 cells were pre-extracted with 0.5% Triton on ice prior to fixation and immunofluorescence staining. (Top) Representative field of cells in mid-S phase treated with or without HU for 2 hr. Scale bar, 20 mm. ...View More
Jasencakova, Z., Scharf, A. N., Ask, K., Corpet, A., Imhof, A., Almouzni, G., & Groth, A. (2010). Replication stress interferes with histone recycling and predeposition marking of new histones. Molecular cell, 37(5), 736-743.
Rabbit Anti-ASF1B Recombinant Antibody (EG272) CBMAB-EN300-LY in WB
ASF1B protein expression was detected by western blotting. ...View More
Zhang, Z., Ning, M., Li, L., Li, Z., Wang, Y., & Zhao, J. (2023). Knockdown of ASF1B inhibits cell proliferation, migration, invasion and cisplatin resistance in gastric cancer through the Myc pathway. Oncology Letters, 25(6), 1-11.
Rabbit Anti-ASF1B Recombinant Antibody (EG272) CBMAB-EN300-LY in Immunoblot
Whole cell lysates (WCL), cytoplasm, nuclear, and chromatin fractions of A549 cells transduced with the indicated sgRNAs were analyzed by immunoblots. ...View More
Xie, Y., Sahin, M., Wakamatsu, T., Inoue-Yamauchi, A., Zhao, W., Han, S., ... & Cheng, E. H. (2023). SETD2 regulates chromatin accessibility and transcription to suppress lung tumorigenesis. JCI insight, 8(4).

Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
EG272
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding the the carboxy-terminus of the human ASF1B protein.
Specificity
Human, Monkey
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10mM HEPES, pH 7.5, 0.15M NaCl, 0.1mg/mL BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Anti-Silencing Function 1B Histone Chaperone
Introduction
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human55723
Mouse66929
Rat304648
UniProt ID
HumanQ9NVP2
MouseQ9DAP7
RatB0BN69
Alternative Names
Anti-Silencing Function 1B Histone Chaperone; Anti-Silencing Function Protein 1 Homolog B; CCG1-Interacting Factor A-II; HCIA-II; CIA-II; HAsf1b;
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.
Biological Process
Blastocyst hatching Source: Ensembl
Cell differentiation Source: UniProtKB-KW
DNA replication-dependent nucleosome assembly Source: UniProtKB
DNA replication-independent nucleosome assembly Source: UniProtKB
Spermatogenesis Source: UniProtKB-KW
Cellular Location
Nucleus
PTM
Phosphorylated by TLK1 and TLK2.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-ASF1B Recombinant Antibody (EG272)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare