Mouse Anti-ASF1B Recombinant Antibody (CBYC-A818) (CBMAB-A3760-YC)

Name: Mouse Anti-ASF1B Recombinant Antibody (CBYC-A818)
SKU: CBMAB-A3760-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A818

Application

ELISA, WB, IF

Immunogen

ASF1B (AAH36521, 1 a.a. ~ 202 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Anti-Silencing Function 1B Histone Chaperone

Introduction

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key r

Entrez Gene ID

55723

UniProt ID

Q9NVP2

Alternative Names

Anti-Silencing Function 1B Histone Chaperone; Anti-Silencing Function Protein 1 Homolog B; CCG1-Interacting Factor A-II; HCIA-II; CIA-II; HAsf1b;

Function

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.

Biological Process

Blastocyst hatching Source: Ensembl
Cell differentiation Source: UniProtKB-KW
DNA replication-dependent nucleosome assembly Source: UniProtKB
DNA replication-independent nucleosome assembly Source: UniProtKB
Spermatogenesis Source: UniProtKB-KW

Cellular Location

Nucleus

PTM

Phosphorylated by TLK1 and TLK2.

Jiangqiao, Z., Tao, Q., Zhongbao, C., Xiaoxiong, M., Long, Z., Jilin, Z., & Tianyu, W. (2019). Anti-silencing function 1B histone chaperone promotes cell proliferation and migration via activation of the AKT pathway in clear cell renal cell carcinoma. Biochemical and biophysical research communications, 511(1), 165-172.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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