Rabbit Anti-BMI1 Recombinant Antibody (
1F2) (V2LY-0725-LY1467)

Name: Rabbit Anti-BMI1 Recombinant Antibody (1F2)
SKU: V2LY-0725-LY1467
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-BMI1 Recombinant Antibody (1F2) (V2LY-0725-LY1467) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, U87 whole cell lysate, U251 whole cell lysate
All lanes: BMI1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa

Rabbit Anti-BMI1 Recombinant Antibody (1F2) (V2LY-0725-LY1467) in IHC

IHC image of V2LY-0725-LY1467 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-BMI1 Recombinant Antibody (1F2) (V2LY-0725-LY1467) in IHC

IHC image of V2LY-0725-LY1467 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

1F2

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human Bmi1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

BMI1 Proto-Oncogene, Polycomb Ring Finger

Entrez Gene ID

648

UniProt ID

P35226

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373).
The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358).
In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).

Biological Process

Chromatin silencing Source: GO_Central
Hemopoiesis Source: UniProtKB
Histone H2A-K119 monoubiquitination Source: UniProtKB
Negative regulation of G0 to G1 transition Source: Reactome
Negative regulation of gene expression, epigenetic Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of fibroblast proliferation Source: BHF-UCL
Positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
Regulation of gene expression Source: BHF-UCL
Segment specification Source: ProtInc

Cellular Location

Nucleus; Cytoplasm

PTM

Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

Fitieh, A., Locke, A. J., Motamedi, M., & Ismail, I. H. (2021). The Role of Polycomb Group Protein BMI1 in DNA Repair and Genomic Stability. International Journal of Molecular Sciences, 22(6), 2976.

Wu, D., He, X., Wang, W., Hu, X., Wang, K., & Wang, M. (2020). Long noncoding RNA SNHG12 induces proliferation, migration, epithelial–mesenchymal transition, and stemness of esophageal squamous cell carcinoma cells via post‐transcriptional regulation of BMI1 and CTNNB1. Molecular oncology, 14(9), 2332-2351.

Solorzano-Vargas, R. S., Bjerknes, M., Wu, S. V., Wang, J., Stelzner, M., Dunn, J. C., ... & Martín, M. G. (2019). The cellular regulators PTEN and BMI1 help mediate NEUROGENIN-3–induced cell cycle arrest. Journal of Biological Chemistry, 294(41), 15182-15192.

Wu, L., Zhang, D., Zhou, L., Pei, Y., Zhuang, Y., Cui, W., & Chen, J. (2019). FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis. EBioMedicine, 41, 384-394.

Li, P., Liu, D., Mei, Q., Wang, X., Zheng, Y., Pan, H., & Zheng, L. (2018, June). Research on the Effect of BMI1 Gene Silencing on the Proliferation Activity of Human Breast Cancer Cells. In 2018 8th International Conference on Mechatronics, Computer and Education Informationization (MCEI 2018) (pp. 88-93). Atlantis Press.

Vaishnave, S. (2018). BMI1 and PTEN are key determinants of breast cancer therapy: A plausible therapeutic target in breast cancer. Gene, 678, 302-311.

Koh, H., Park, H., Chandimali, N., Huynh, D. L., Zhang, J. J., Ghosh, M., ... & Jeong, D. K. (2017). MicroRNA-128 suppresses paclitaxel-resistant lung cancer by inhibiting MUC1-C and BMI-1 in cancer stem cells. Oncotarget, 8(66), 110540.

Liu, X., Wei, W., Li, X., Shen, P., Ju, D., Wang, Z., ... & Xi, R. (2017). BMI1 and MEL18 promote colitis-associated cancer in mice via REG3B and STAT3. Gastroenterology, 153(6), 1607-1620.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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