Mouse Anti-Bmi1 Recombinant Antibody (CBYY-1829) (CBMAB-2178-YY)

Name: Mouse Anti-Bmi1 Recombinant Antibody (CBYY-1829)
SKU: CBMAB-2178-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-1829

Application

IF, IH, IC, WB, IP

Immunogen

BMI1 Fusion Protein (1-326 aa).

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000
IHC	1:20-1:200
IF(ICC)	1:10-1:100
IP	0.5-4.0 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BMI1 Proto-Oncogene, Polycomb Ring Finger

Introduction

This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.

Entrez Gene ID

648

UniProt ID

P35226

Alternative Names

BMI1 Proto-Oncogene, Polycomb Ring Finger; Polycomb Group RING Finger Protein 4; PCGF4; RNF51; B Lymphoma Mo-MLV Insertion Region 1 Homolog (Mouse); Murine Leukemia Viral (Bmi-1) Oncogene Homolog; B Lymphoma Mo-MLV Insertion Region 1 Homolog; BMI1 Polycomb Ring Finger Proto-Oncogene; BMI1 Polycomb Ring Finger Oncogene;

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373).
The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358).
In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).

Biological Process

Chromatin silencing Source: GO_Central
Hemopoiesis Source: UniProtKB
Histone H2A-K119 monoubiquitination Source: UniProtKB
Negative regulation of G0 to G1 transition Source: Reactome
Negative regulation of gene expression, epigenetic Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of fibroblast proliferation Source: BHF-UCL
Positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
Regulation of gene expression Source: BHF-UCL
Segment specification Source: ProtInc

Cellular Location

Nucleus; Cytoplasm

PTM

Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

Fitieh, A., Locke, A. J., Motamedi, M., & Ismail, I. H. (2021). The Role of Polycomb Group Protein BMI1 in DNA Repair and Genomic Stability. International Journal of Molecular Sciences, 22(6), 2976.

Wu, D., He, X., Wang, W., Hu, X., Wang, K., & Wang, M. (2020). Long noncoding RNA SNHG12 induces proliferation, migration, epithelial–mesenchymal transition, and stemness of esophageal squamous cell carcinoma cells via post‐transcriptional regulation of BMI1 and CTNNB1. Molecular oncology, 14(9), 2332-2351.

Solorzano-Vargas, R. S., Bjerknes, M., Wu, S. V., Wang, J., Stelzner, M., Dunn, J. C., ... & Martín, M. G. (2019). The cellular regulators PTEN and BMI1 help mediate NEUROGENIN-3–induced cell cycle arrest. Journal of Biological Chemistry, 294(41), 15182-15192.

Wu, L., Zhang, D., Zhou, L., Pei, Y., Zhuang, Y., Cui, W., & Chen, J. (2019). FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis. EBioMedicine, 41, 384-394.

Li, P., Liu, D., Mei, Q., Wang, X., Zheng, Y., Pan, H., & Zheng, L. (2018, June). Research on the Effect of BMI1 Gene Silencing on the Proliferation Activity of Human Breast Cancer Cells. In 2018 8th International Conference on Mechatronics, Computer and Education Informationization (MCEI 2018) (pp. 88-93). Atlantis Press.

Vaishnave, S. (2018). BMI1 and PTEN are key determinants of breast cancer therapy: A plausible therapeutic target in breast cancer. Gene, 678, 302-311.

Koh, H., Park, H., Chandimali, N., Huynh, D. L., Zhang, J. J., Ghosh, M., ... & Jeong, D. K. (2017). MicroRNA-128 suppresses paclitaxel-resistant lung cancer by inhibiting MUC1-C and BMI-1 in cancer stem cells. Oncotarget, 8(66), 110540.

Liu, X., Wei, W., Li, X., Shen, P., Ju, D., Wang, Z., ... & Xi, R. (2017). BMI1 and MEL18 promote colitis-associated cancer in mice via REG3B and STAT3. Gastroenterology, 153(6), 1607-1620.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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