Mouse Anti-CA3 Recombinant Antibody (CBYY-C0254) (CBMAB-C0740-YY)

Name: Mouse Anti-CA3 Recombinant Antibody (CBYY-C0254)
SKU: CBMAB-C0740-YY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBYY-C0254

Antibody Isotype

IgG3, κ

Application

E, WB, IF, P, IP

Basic Information

Immunogen

Amino acids 27-55 of CA3 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG3, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Entrez Gene ID

761

UniProt ID

P07451

Alternative Names

Killer Cell Lectin Like Receptor B1; Natural Killer Cell Surface Protein P1A; Killer Cell Lectin-Like Receptor Subfamily B, Member 1; C-Type Lectin Domain Family 5 Member B; HNKR-P1A; NKR-P1A; CLEC5B;

Function

Reversible hydration of carbon dioxide.

Biological Process

Bicarbonate transport Source: Reactome
One-carbon metabolic process Source: GO_Central
Response to bacterium Source: Ensembl
Response to ethanol Source: Ensembl
Response to oxidative stress Source: Ensembl

Cellular Location

Cytoplasm

PTM

S-glutathionylated in hepatocytes under oxidative stress.

More Infomation

References

Liu, D., Wong, C. C., Zhou, Y., Li, C., Chen, H., Ji, F., ... & Yu, J. (2021). Squalene Epoxidase Induces Nonalcoholic Steatohepatitis Via Binding to Carbonic Anhydrase III and is a Therapeutic Target. Gastroenterology, 160(7), 2467-2482.

NG, B., & Budak, H. (2021). Tip60 Might Be a Candidate For The Acetylation of Hepatic Carbonic Anhydrase I and III in Mice.

BALTACI, N. G., KOCPINAR, E. F., & Budak, H. (2021). Tip60 Might Be a Candidate For The Acetylation of Hepatic Carbonic Anhydrase I and III in Mice.

Aspatwar, A., Hammaren, M., Parikka, M., Parkkila, S., Carta, F., Bozdag, M., ... & Supuran, C. T. (2020). In vitro inhibition of Mycobacterium tuberculosis β-carbonic anhydrase 3 with Mono-and dithiocarbamates and evaluation of their toxicity using zebrafish developing embryos. Journal of enzyme inhibition and medicinal chemistry, 35(1), 65-71.

Barhwal, K. K., Biswal, S., Nag, T. C., Chaurasia, O. P., & Hota, S. K. (2020). Class switching of carbonic anhydrase isoforms mediates remyelination in CA3 hippocampal neurons during chronic hypoxia. Free Radical Biology and Medicine, 161, 102-114.

Roy, P., Ireland, M., Roy, S., Craft, J., & Bartholomew, C. (2018). Carbonic Anhydrase III S-Glutathionylation Is Necessary for Anti-Oxidant Activity. Yangtze Medicine, 2(04), 244.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

CA3 Matched Antibody Pair (164) (CAT#: APMAB-164LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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