Human Recombinant CA3 protein, His Tag (V2LY-0526-LY2551)

Name: Human Recombinant CA3 protein, His Tag
SKU: V2LY-0526-LY2551
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CA3 protein, His Tag consist of Amino Acid: 1-260 and predicts a molecular mass of 30.4 kDa.

Molecule Mass

30.4 kDa

Sequence

Amino Acid: 1-260

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, 0.1% Brij35

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CA3

Function

Reversible hydration of carbon dioxide.

Biological Process

Bicarbonate transport Source: Reactome
One-carbon metabolic process Source: GO_Central
Response to bacterium Source: Ensembl
Response to ethanol Source: Ensembl
Response to oxidative stress Source: Ensembl

Cellular Location

Cytoplasm

PTM

S-glutathionylated in hepatocytes under oxidative stress.

Liu, D., Wong, C. C., Zhou, Y., Li, C., Chen, H., Ji, F., ... & Yu, J. (2021). Squalene Epoxidase Induces Nonalcoholic Steatohepatitis Via Binding to Carbonic Anhydrase III and is a Therapeutic Target. Gastroenterology, 160(7), 2467-2482.

NG, B., & Budak, H. (2021). Tip60 Might Be a Candidate For The Acetylation of Hepatic Carbonic Anhydrase I and III in Mice.

BALTACI, N. G., KOCPINAR, E. F., & Budak, H. (2021). Tip60 Might Be a Candidate For The Acetylation of Hepatic Carbonic Anhydrase I and III in Mice.

Aspatwar, A., Hammaren, M., Parikka, M., Parkkila, S., Carta, F., Bozdag, M., ... & Supuran, C. T. (2020). In vitro inhibition of Mycobacterium tuberculosis β-carbonic anhydrase 3 with Mono-and dithiocarbamates and evaluation of their toxicity using zebrafish developing embryos. Journal of enzyme inhibition and medicinal chemistry, 35(1), 65-71.

Barhwal, K. K., Biswal, S., Nag, T. C., Chaurasia, O. P., & Hota, S. K. (2020). Class switching of carbonic anhydrase isoforms mediates remyelination in CA3 hippocampal neurons during chronic hypoxia. Free Radical Biology and Medicine, 161, 102-114.

Roy, P., Ireland, M., Roy, S., Craft, J., & Bartholomew, C. (2018). Carbonic Anhydrase III S-Glutathionylation Is Necessary for Anti-Oxidant Activity. Yangtze Medicine, 2(04), 244.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CA3 Matched Antibody Pair (164) (CAT#: APMAB-164LY)

Antibody Pairs

CA3 Matched Antibody Pair (164) (CAT#: APMAB-164LY)

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CA3 Matched Antibody Pair (164) (CAT#: APMAB-164LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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