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Mouse Anti-CASP7 Recombinant Antibody (CBFYC-0849) (CBMAB-C0904-FY)


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Published Data
CBMAB-C0904-FY-1
Mouse Anti-CASP7 Recombinant Antibody (CBFYC-0849) (CBMAB-C0904-FY) in Immunoblot
Cells pretreated with or without 0.5 μM C16 for 1 h were treated with the indicated concentrations of doxorubicin for 32 h and analyzed via immunoblotting using specific antibodies against PARP, c-Cas-7, and β-actin. Arrowhead indicates cleaved form of PARP-1. ...View More
Lee, S., Jee, H. Y., Lee, Y. G., Shin, J. I., Jeon, Y. J., Kim, J. B., ... & Lee, K. (2022). PKR-mediated phosphorylation of eIF2a and CHK1 is associated with doxorubicin-mediated apoptosis in HCC1143 triple-negative breast cancer cells. International Journal of Molecular Sciences, 23(24), 15872.
CBMAB-C0904-FY-2
Mouse Anti-CASP7 Recombinant Antibody (CBFYC-0849) (CBMAB-C0904-FY) in WB
Comparison of protein expression from NK3.3-derived EVs (3.3EV), NK3.3 cells (NK3.3lys), or K562 cells 24 h post- 100 μg/ml NK3.3 EV treatment (K562lys). Lysates were prepared, and immunoblotting performed to assess the potential contribution of NK EV-associated proteins to NK EV-treated K562 cells (25 μg of protein per lane). ...View More
Cochran, A. M., & Kornbluth, J. (2021). Extracellular vesicles from the human natural killer cell line NK3. 3 have broad and potent anti-tumor activity. Frontiers in Cell and Developmental Biology, 9, 698639.
CBMAB-C0904-FY-3
Mouse Anti-CASP7 Recombinant Antibody (CBFYC-0849) (CBMAB-C0904-FY) in IHC
Representative photomicrographs of the equine placenta retrieved from CRL, NPN, NPL, and immunostained for S100A9, CASP7, HIF1A, VEGF, TBX21, and CYP11A1. Bar = 100 µm. Key; CRL; normal postpartum (control), NPL; nocardioform placentitis lesion, and NPN; nocardioform placentitis normal region, CASP7; caspase 7, HIF1A; hypoxia inducible Factor 1 subunit alpha, VEGF; vascular endothelial growth factor, CYP11A1; cytochrome P450 family 11 subfamily A member 1. ...View More
El-Sheikh Ali, H., Loux, S. C., Kennedy, L., Scoggin, K. E., Dini, P., Fedorka, C. E., ... & Ball, B. A. (2021). Transcriptomic analysis of equine chorioallantois reveals immune networks and molecular mechanisms involved in nocardioform placentitis. Veterinary research, 52, 1-22.
CBMAB-C0904-FY-4
Mouse Anti-CASP7 Recombinant Antibody (CBFYC-0849) (CBMAB-C0904-FY) in WB
HCT116 and RKO cells were treated by 10 μM of Sur-X (0.5, 1, 3 and 6 h) or Con (6 h), the expression levels of apoptosis-related proteins were detected by Western blot analysis. GAPDH was used as a loading control. NT, no treatment. Three independent experiments were performed. ...View More
Fang, W., Che, X., Li, G., Wang, A., Wang, Y., Shi, X., ... & Liu, Y. (2020). Sur-X, a novel peptide, kills colorectal cancer cells by targeting survivin-XIAP complex. Journal of Experimental & Clinical Cancer Research, 39, 1-18.
CBMAB-C0904-FY-5
Mouse Anti-CASP7 Recombinant Antibody (CBFYC-0849) (CBMAB-C0904-FY) in WB
Expression of the cleaved form and the total level of caspase-7 and PARP1 were detected by Western blotting. ...View More
Zhang, J., Yang, S., Xu, B., Wang, T., Zheng, Y., Liu, F., ... & Dong, L. (2019). p62 functions as an oncogene in colorectal cancer through inhibiting apoptosis and promoting cell proliferation by interacting with the vitamin D receptor. Cell proliferation, 52(3), e12585.
CBMAB-C0904-FY-6
Mouse Anti-CASP7 Recombinant Antibody (CBFYC-0849) (CBMAB-C0904-FY) in WB
Western blot analysis to evaluate CASP3 and CASP7 protein levels in miR-224 overexpressing H1299 and H3122 lung cancer cells. ...View More
Cui, R., Kim, T., Fassan, M., Meng, W., Sun, H. L., Jeon, Y. J., ... & Croce, C. M. (2015). MicroRNA-224 is implicated in lung cancer pathogenesis through targeting caspase-3 and caspase-7. Oncotarget, 6(26), 21802.
CBMAB-C0904-FY-7
Mouse Anti-CASP7 Recombinant Antibody (CBFYC-0849) (CBMAB-C0904-FY) in IHC
Cell migration assay for CASP7 knockdown H1299 cells using transwell membranes. Representative pictures were taken under 100 times magnification. The average counts were derived from six random microscopic fields. ...View More
Cui, R., Kim, T., Fassan, M., Meng, W., Sun, H. L., Jeon, Y. J., ... & Croce, C. M. (2015). MicroRNA-224 is implicated in lung cancer pathogenesis through targeting caspase-3 and caspase-7. Oncotarget, 6(26), 21802.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Horse
Clone
CBFYC-0849
Antibody Isotype
IgG1, κ
Application
ELISA, IHC-P, IF, IP, WB

Basic Information

Immunogen
Amino acids 15-50 near the N-terminus of caspase-7 p20 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Horse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Caspase 7
Introduction
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Entrez Gene ID
Human840
Mouse12369
Rat64026
Monkey697633
UniProt ID
HumanP55210
MouseP97864
RatO88550
MonkeyA0A2K5VRV4
Alternative Names
Caspase 7; Caspase 7, Apoptosis-Related Cysteine Peptidase; Caspase 7, Apoptosis-Related Cysteine Protease; ICE-Like Apoptotic Protease 3; ICE-LAP3; CASP-7; CMH-1; MCH3
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.
Biological Process
Apoptotic process Source: UniProtKB
Cellular response to staurosporine Source: CAFA
Execution phase of apoptosis Source: GO_Central
Proteolysis Source: UniProtKB
Cellular Location
Cytoplasm
PTM
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
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For research use only. Not intended for any clinical use.

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