Mouse Anti-CASP7 Recombinant Antibody (CBYY-C0982) (CBMAB-C2419-YY)

Name: Mouse Anti-CASP7 Recombinant Antibody (CBYY-C0982)
SKU: CBMAB-C2419-YY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBYY-C0982

Antibody Isotype

IgG1, κ

Application

ELISA, IF, IP, WB

Basic Information

Immunogen

Amino acids 257-281 near the C-terminus of caspase-7 p10 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Caspase 7

Entrez Gene ID

840

UniProt ID

P55210

Alternative Names

Ciliogenesis Associated TTC17 Interacting Protein; C2orf62; Ciliogenesis-Associated TTC17-Interacting Protein; Chromosome 2 Open Reading Frame 62;

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.

Biological Process

Apoptotic process Source: UniProtKB
Cellular response to staurosporine Source: CAFA
Execution phase of apoptosis Source: GO_Central
Proteolysis Source: UniProtKB

Cellular Location

Cytoplasm

PTM

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.

More Infomation

References

Mahib, M. R., Hosojima, S., Kushiyama, H., Kinoshita, T., Shiroishi, T., Suda, T., & Tsuchiya, K. (2020). Caspase‐7 mediates caspase‐1‐induced apoptosis independently of Bid. Microbiology and immunology, 64(2), 143-152.

Safdar, M., Zaheer, S., Khailany, R. A., Parvez, S., Naveed, M., Bhuiyan, P., ... & Junejo, Y. (2020). The Relevance of SNPs at 3′ UTR Region of CASP7 and miR-371b-5p Associated Diseases: A Computational Analysis. Cell Biochemistry and Biophysics, 78(4), 541-557.

Gonçalves, A. V., Margolis, S. R., Quirino, G. F., Mascarenhas, D. P., Rauch, I., Nichols, R. D., ... & Zamboni, D. S. (2019). Gasdermin-D and Caspase-7 are the key Caspase-1/8 substrates downstream of the NAIP5/NLRC4 inflammasome required for restriction of Legionella pneumophila. PLoS pathogens, 15(6), e1007886.

Pham, B., Eron, S. J., Hill, M. E., Li, X., Fahie, M. A., Hardy, J. A., & Chen, M. (2019). A nanopore approach for analysis of caspase-7 activity in cell lysates. Biophysical journal, 117(5), 844-855.

Shigemura, M., Lecuona, E., Angulo, M., Homma, T., Rodríguez, D. A., Gonzalez-Gonzalez, F. J., ... & Sznajder, J. I. (2018). Hypercapnia increases airway smooth muscle contractility via caspase-7–mediated miR-133a–RhoA signaling. Science translational medicine, 10(457).

Venkatesh, A., Cheng, S. Y., & Punzo, C. (2017). Loss of the cone-enriched caspase-7 does not affect secondary cone death in retinitis pigmentosa. Molecular vision, 23, 944.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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