Mouse Anti-CASP7 Recombinant Antibody (CBT2044) (V2LY-0625-LY179)

Name: Mouse Anti-CASP7 Recombinant Antibody (CBT2044)
SKU: V2LY-0625-LY179
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CASP7 Recombinant Antibody (CBT2044) (V2LY-0625-LY179) in WB

Mouse Anti-CASP7 Recombinant Antibody (CBT2044) (V2LY-0625-LY179) in FC

Mouse Anti-CASP7 Recombinant Antibody (CBT2044) (V2LY-0625-LY179) in IHC

Mouse Anti-CASP7 Recombinant Antibody (CBT2044) (V2LY-0625-LY179) in ELISA

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT2044

Antibody Isotype

IgG1

Application

WB, IHC, FC

Basic Information

Immunogen

Purified recombinant fragment of human CASP-7 (AA: 29-198) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID

840

UniProt ID

P55210

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.

Biological Process

Apoptotic process Source: UniProtKB
Cellular response to staurosporine Source: CAFA
Execution phase of apoptosis Source: GO_Central
Proteolysis Source: UniProtKB

Cellular Location

Cytoplasm

PTM

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.

More Infomation

References

Mahib, M. R., Hosojima, S., Kushiyama, H., Kinoshita, T., Shiroishi, T., Suda, T., & Tsuchiya, K. (2020). Caspase‐7 mediates caspase‐1‐induced apoptosis independently of Bid. Microbiology and immunology, 64(2), 143-152.

Safdar, M., Zaheer, S., Khailany, R. A., Parvez, S., Naveed, M., Bhuiyan, P., ... & Junejo, Y. (2020). The Relevance of SNPs at 3′ UTR Region of CASP7 and miR-371b-5p Associated Diseases: A Computational Analysis. Cell Biochemistry and Biophysics, 78(4), 541-557.

Gonçalves, A. V., Margolis, S. R., Quirino, G. F., Mascarenhas, D. P., Rauch, I., Nichols, R. D., ... & Zamboni, D. S. (2019). Gasdermin-D and Caspase-7 are the key Caspase-1/8 substrates downstream of the NAIP5/NLRC4 inflammasome required for restriction of Legionella pneumophila. PLoS pathogens, 15(6), e1007886.

Pham, B., Eron, S. J., Hill, M. E., Li, X., Fahie, M. A., Hardy, J. A., & Chen, M. (2019). A nanopore approach for analysis of caspase-7 activity in cell lysates. Biophysical journal, 117(5), 844-855.

Shigemura, M., Lecuona, E., Angulo, M., Homma, T., Rodríguez, D. A., Gonzalez-Gonzalez, F. J., ... & Sznajder, J. I. (2018). Hypercapnia increases airway smooth muscle contractility via caspase-7–mediated miR-133a–RhoA signaling. Science translational medicine, 10(457).

Venkatesh, A., Cheng, S. Y., & Punzo, C. (2017). Loss of the cone-enriched caspase-7 does not affect secondary cone death in retinitis pigmentosa. Molecular vision, 23, 944.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote
Second antibody and isotype control

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