Rabbit Anti-CBX1 Recombinant Antibody (D2F2) (CBMAB-H0017-FY)

Name: Rabbit Anti-CBX1 Recombinant Antibody (D2F2)
SKU: CBMAB-H0017-FY
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-CBX1 Recombinant Antibody (D2F2) (CBMAB-H0017-FY) in IP

Endogenous Cbx1 and PurB was immunoprecipitated from cardiac fibroblast cell lysates. An isotype control antibody was used as a control. Immunoprecipitates were immunoblotted with a Cbx1, PurB, and a Sp3 antibody. The first lane contains cell extract (1/10th immunoprecipitation input). Representative immunoblots are shown from three independent experiments. ...View More

Baksh, S. S., Pratt, R. E., Gomez, J., Dzau, V. J., & Hodgkinson, C. P. (2022). A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue-and lineage-specific genes. Journal of Biological Chemistry, 298(6).

Rabbit Anti-CBX1 Recombinant Antibody (D2F2) (CBMAB-H0017-FY) in Immunoblot

Immunoblot analyses of ASH1L and HP1β in CENP- B WT and CENP-B KO cell line. The antibodies used are indicated on the left. No significant difference was detected in total protein levels of ASH1L and HP1β between CENP-B WT and KO cell lines. ...View More

Otake, K., Ohzeki, J. I., Shono, N., Kugou, K., Okazaki, K., Nagase, T., ... & Masumoto, H. (2020). CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly. Journal of cell science, 133(15), jcs243303.

Rabbit Anti-CBX1 Recombinant Antibody (D2F2) (CBMAB-H0017-FY) in WB

Immobilized H3K9me templates according to the pull-down experimental schemes were incubated with recombinant hHP1β WT. Material recovered after washing was analysed by western blotting. ...View More

Hiragami-Hamada, K., Soeroes, S., Nikolov, M., Wilkins, B., Kreuz, S., Chen, C., ... & Fischle, W. (2016). Dynamic and flexible H3K9me3 bridging via HP1β dimerization establishes a plastic state of condensed chromatin. Nature communications, 7(1), 11310.

Rabbit Anti-CBX1 Recombinant Antibody (D2F2) (CBMAB-H0017-FY) in IF

SMARCA4MUT (A549 and H1299) cells transduced with EV or SMARCA4 were immunostained with anti-HP1b antibody, and the nuclei were counterstained with DAPI. Scale bar: 10 um. ...View More

Kurashima, K., Kashiwagi, H., Shimomura, I., Suzuki, A., Takeshita, F., Mazevet, M., ... & Shiotani, B. (2020). SMARCA4 deficiency-associated heterochromatin induces intrinsic DNA replication stress and susceptibility to ATR inhibition in lung adenocarcinoma. NAR cancer, 2(2), zcaa005.

Rabbit Anti-CBX1 Recombinant Antibody (D2F2) (CBMAB-H0017-FY) in IF

ASFV modifies the host chromatin state. Immunofluorescence analysis was performed on non-infected and ASFV-infected Vero cells (8 hpi) using specific antibodies recognizing heterochromatin marks-H3K9me3, HP1β and HDAC2 (red) and viral proteins (green). Cell nuclei were counterstained with DAPI (blue). ...View More

Simões, M., Rino, J., Pinheiro, I., Martins, C., & Ferreira, F. (2015). Alterations of nuclear architecture and epigenetic signatures during African swine fever virus infection. Viruses, 7(9), 4978-4996.

Rabbit Anti-CBX1 Recombinant Antibody (D2F2) (CBMAB-H0017-FY) in WB

Control cells were transfected with 1 nM of two independent siRNAs of HP1α and HP1β for 24 h. The expression level of HP1α and HP1β were analyzed by western blotting. ...View More

Igarashi, T., Mazevet, M., Yasuhara, T., Yano, K., Mochizuki, A., Nishino, M., ... & Shiotani, B. (2023). An ATR-PrimPol pathway confers tolerance to oncogenic KRAS-induced and heterochromatin-associated replication stress. Nature Communications, 14(1), 4991.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D2F2

Application

WB, IP, IF

Immunogen

Synthetic peptide corresponding to residues surrounding Gln69 of human HP1β protein.

Host Species

Rabbit

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:50
IF(ICC)	1:800

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Chromobox 1

Introduction

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Entrez Gene ID

Human	10951
Mouse	12412
Monkey	696673
Hamster	100689359
Rat	360609
Cattle	616506
Guinea pig	100716439

UniProt ID

Human	P83916
Mouse	P83917
Rat	D4A3T3
Monkey	A0A0D9S3Q7
Hamster	Q6E6J7
Cattle	A0A4W2F6Q7
Guinea pig	A0A286XV31

Alternative Names

Chromobox 1; Heterochromatin Protein 1 Homolog Beta; Modifier 1 Protein; HP1Hsbeta; P25beta; CBX; M31; Chromobox Homolog 1 (HP1 Beta Homolog Drosophila ); Chromobox Homolog 1 (Drosophila HP1 Beta); Heterochromatin Protein P25 Beta; HP1 Beta Homolog (Drosophila )

Function

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.

Biological Process

Cellular response to DNA damage stimulus Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: Ensembl
Viral life cycle Source: Reactome

Cellular Location

Nucleus. Unassociated with chromosomes during mitosis.

PTM

Not phosphorylated.
Ubiquitinated.

Hu, A., Zhang, Y., Zhao, X., Li, J., & Ying, Y. (2019). CBX1 is a direct target of miR-205-5p and contributes to the progression of pituitary tumor. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 74(3), 154-156.

Wang, H., Zhang, X. M., Tomiyoshi, G. O., Nakamura, R., Shinmen, N., Kuroda, H., ... & Hiwasa, T. (2018). Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction. Oncotarget, 9(5), 5600.

Yang, Y. F., Pan, Y. H., Tian, Q. H., Wu, D. C., & Su, S. G. (2018). CBX1 indicates poor outcomes and exerts oncogenic activity in hepatocellular carcinoma. Translational oncology, 11(5), 1110-1118.

Ning, G., Huang, Y. L., Zhen, L. M., Xu, W. X., Jiao, Q., Yang, F. J., ... & Peng, L. (2018). Transcriptional expressions of Chromobox 1/2/3/6/8 as independent indicators for survivals in hepatocellular carcinoma patients. Aging (Albany NY), 10(11), 3450.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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