Rat Anti-CFD Recombinant Antibody (C2405) (CBMAB-C2405-LY)

Name: Rat Anti-CFD Recombinant Antibody (C2405)
SKU: CBMAB-C2405-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

C2405

Application

IP, WB

Immunogen

Recombinant mouse Complement Factor D/Adipsin Ile26-Ser259.

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	2 μg/ml
IP	25 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, 5% trehalose

Preservative

None

Concentration

0.5 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Complement Factor D

Introduction

This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease. [provided by RefSeq, Nov 2015]

Entrez Gene ID

11537

UniProt ID

P03953

Function

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Biological Process

Complement activation Source: ProtInc
Complement activation, alternative pathway Source: Reactome
Neutrophil degranulation Source: Reactome
Notch signaling pathway Source: InterPro
Platelet degranulation Source: Reactome
Proteolysis Source: ProtInc

Cellular Location

Secreted

Involvement in disease

Complement factor D deficiency (CFDD): An immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway.

Duan, C., Ma, Z., Wu, S., Ding, X., Tu, X., & Ye, J. (2021). Functional characterization of complement factor D on the defense against Gyrodactylus kobayashii (Monogenea) infection in goldfish (Carassius auratus). Aquaculture, 545, 737214.

Corvillo, F., González-Sánchez, L., López-Lera, A., Arjona, E., Ceccarini, G., Santini, F., ... & López-Trascasa, M. (2021). Complement Factor D (adipsin) Levels Are Elevated in Acquired Partial Lipodystrophy (Barraquer–Simons syndrome). International Journal of Molecular Sciences, 22(12), 6608.

Langereis, J. D., van der Molen, R. G., de Kat Angelino, C., Henriet, S. S., de Jonge, M. I., Joosten, I., ... & van der Flier, M. (2021). Complement factor D haplodeficiency is associated with a reduced complement activation speed and diminished bacterial killing. Clinical & Translational Immunology, 10(4), e1256.

Wiles, J. A., Galvan, M. D., Podos, S. D., Geffner, M., & Huang, M. (2020). Discovery and development of the oral complement factor D inhibitor danicopan (ACH-4471). Current medicinal chemistry, 27(25), 4165-4180.

Qi, H., Wei, J., Gao, Y., Yang, Y., Li, Y., Zhu, H., ... & Yang, R. (2020). Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut. Communications biology, 3(1), 1-15.

Cho, H. Y., Park, H. S., Ko, E. J., Ryu, C. S., Kim, J. O., Kim, Y. R., ... & Kim, N. K. (2020). Association of complement factor D and H polymorphisms with recurrent pregnancy loss. International journal of molecular sciences, 21(1), 17.

Ezure, T., Sugahara, M., & Amano, S. (2019). Senescent dermal fibroblasts negatively influence fibroblast extracellular matrix‐related gene expression partly via secretion of complement factor D. BioFactors, 45(4), 556-562.

Yaspan, B. L., Williams, D. F., Holz, F. G., Regillo, C. D., Li, Z., Dressen, A., ... & MAHALO Study Investigators. (2017). Targeting factor D of the alternative complement pathway reduces geographic atrophy progression secondary to age-related macular degeneration. Science translational medicine, 9(395).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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