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Mouse Anti-DHX8 Recombinant Antibody (A2221) (V2LY-0425-LY298)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
A2221
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 446-587 mapping within an internal region of human DDX8.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DEAH-Box Helicase 8
Entrez Gene ID
Human1659
Mouse217207
UniProt ID
HumanQ14562
MouseA2A4P0
Function
Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28502770, PubMed:28076346).

Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome (PubMed:8608946).
Biological Process
mRNA splicing, via spliceosome Source: UniProtKB
RNA processing Source: ProtInc
RNA splicing Source: ProtInc
Spliceosomal complex disassembly Source: GO_Central
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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