Mouse Anti-DNAJB4 Recombinant Antibody (EG977) (V2LY-0425-LY635)

Name: Mouse Anti-DNAJB4 Recombinant Antibody (EG977)
SKU: V2LY-0425-LY635
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DNAJB4 Recombinant Antibody (EG977) (V2LY-0425-LY635-1) in WB

Western blot for monitoring the expression levels of DNAJB4 in HEK293T cells or the isogenic cells with the YTHDF1, YTHDF2, and YTHDF3 genes being ablated by the CRISPR-Cas9 genome editing method at different time intervals following exposure with 100nM ganetespib. ...View More

Miao, W., Li, L., Zhao, Y., Dai, X., Chen, X., & Wang, Y. (2019). HSP90 inhibitors stimulate DNAJB4 protein expression through a mechanism involving N 6-methyladenosine. Nature communications, 10(1), 3613.

Mouse Anti-DNAJB4 Recombinant Antibody (EG977) (V2LY-0425-LY635-2) in WB

Western blot analysis showing the increased protein expression of DNAJB4 in HMLE-Twist cells. ...View More

Kagiali, Z. C. U., Sanal, E., Karayel, Ö., Polat, A. N., Saatci, Ö., Ersan, P. G., ... & Ozlu, N. (2019). Systems-level analysis reveals multiple modulators of epithelial-mesenchymal transition and identifies DNAJB4 and CD81 as novel metastasis inducers in breast cancer. Molecular & Cellular Proteomics, 18(9), 1756-1771.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG977

Application

ELISA, IP, WB

Immunogen

Recombinant human DnaJB4.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DnaJ Heat Shock Protein Family (Hsp40) Member B4

Entrez Gene ID

Human	11080
Mouse	67035
Rat	295549

UniProt ID

Human	Q9UDY4
Mouse	Q9D832
Rat	P33535

Function

Probable chaperone. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).

Biological Process

Chaperone cofactor-dependent protein refolding Source: GO_Central
Response to heat Source: ProtInc
Response to unfolded protein Source: ProtInc

Cellular Location

Cytoplasm; Cell membrane. Cytoplasmic according to PubMed:18837411 and membrane-associated according to PubMed:16542645.

Topf, A., Bengoechea, R., Duff, J., Charlton, R., Mroczek, M., Garcia, S. K., ... & Straub, V. (2021). NEW GENES, NEW TECHNIQUES IN NEUROMUSCULAR DISORDERS: O. 10 Loss of function mutations in DNAJB4 cause a myopathy with early respiratory failure. Neuromuscular Disorders, 31, S160.

Mo, L., Liu, J., Yang, Z., Gong, X., Meng, F., Zou, R., ... & Fang, F. (2020). DNAJB4 identified as a potential breast cancer marker: evidence from bioinformatics analysis and basic experiments. Gland Surgery, 9(6), 1955.

Inoue, M., Iida, A., Watanabe, K., Hosoi, Y., Miyajima, H., Hayashi, S., ... & Nishino, I. (2020). NEW GENES IN NEUROMUSCULAR DISEASES: O. 02 A dominant variant in DNAJB4 causes myofibrillar myopathy. Neuromuscular Disorders, 30, S46.

Miao, W., Li, L., Zhao, Y., Dai, X., Chen, X., & Wang, Y. (2019). HSP90 inhibitors stimulate DNAJB4 protein expression through a mechanism involving N6-methyladenosine. Nature communications, 10(1), 1-12.

Kagiali, Z. C. U., Sanal, E., Karayel, Ö., Polat, A. N., Saatci, Ö., Ersan, P. G., ... & Ozlu, N. (2019). Systems-level Analysis Reveals Multiple Modulators of Epithelial-mesenchymal Transition and Identifies DNAJB4 and CD81 as Novel Metastasis Inducers in Breast Cancer*[S]. Molecular & Cellular Proteomics, 18(9), 1756-1771.

Miao, W., Li, L., & Wang, Y. (2018). A targeted proteomic approach for heat shock proteins reveals DNAJB4 as a suppressor for melanoma metastasis. Analytical chemistry, 90(11), 6835-6842.

Acun, T., Doberstein, N., Habermann, J. K., Gemoll, T., Thorns, C., Oztas, E., & Ried, T. (2017). HLJ1 (DNAJB4) gene is a novel biomarker candidate in breast cancer. Omics: a journal of integrative biology, 21(5), 257-265.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit Anti-DNAJB4 Recombinant Antibody (EG977) (CAT#: CBMAB-EN1159-LY)

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Rabbit Anti-DNAJB4 Recombinant Antibody (EG977) (CAT#: CBMAB-EN1159-LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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