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Mouse Anti-DOT1L Recombinant Antibody (CBYCD-382) (V2LY-0425-LY805)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYCD-382
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-300 mapping at the N-terminus of human DOT1L1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DOT1 Like Histone Lysine Methyltransferase
Entrez Gene ID
Human84444
Mouse208266
Rat362831
UniProt ID
HumanQ8TEK3
MouseQ6XZL8
RatD3ZCP0
Function
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones (PubMed:12123582).

Binds to DNA (PubMed:12628190).
Biological Process
Chromatin silencing at telomere Source: GO_Central
DNA damage checkpoint signaling Source: GO_Central
DNA repair Source: GO_Central
Gene expression Source: Ensembl
Histone H3-K79 methylation Source: MGI
Positive regulation of cell population proliferation Source: MGI
Positive regulation of transcription by RNA polymerase II Source: MGI
Regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Regulation of transcription regulatory region DNA binding Source: UniProtKB
Telomere organization Source: BHF-UCL
Cellular Location
Nucleus
More Infomation

Wu, A., Zhi, J., Tian, T., Cihan, A., Cevher, M. A., Liu, Z., ... & Yu, M. (2021). DOT1L complex regulates transcriptional initiation in human erythroleukemic cells. Proceedings of the National Academy of Sciences, 118(27).

Sarno, F., Nebbioso, A., & Altucci, L. (2020). DOT1L: a key target in normal chromatin remodelling and in mixed-lineage leukaemia treatment. Epigenetics, 15(5), 439-453.

Liu, C., Yang, Q., Zhu, Q., Lu, X., Li, M., Hou, T., ... & Zhu, W. G. (2020). CBP mediated DOT1L acetylation confers DOT1L stability and promotes cancer metastasis. Theranostics, 10(4), 1758.

Anderson, C. J., Baird, M. R., Hsu, A., Barbour, E. H., Koyama, Y., Borgnia, M. J., & McGinty, R. K. (2019). Structural basis for recognition of ubiquitylated nucleosome by Dot1L methyltransferase. Cell reports, 26(7), 1681-1690.

Godfrey, L., Crump, N. T., Thorne, R., Lau, I. J., Repapi, E., Dimou, D., ... & Milne, T. A. (2019). DOT1L inhibition reveals a distinct subset of enhancers dependent on H3K79 methylation. Nature communications, 10(1), 1-15.

Worden, E. J., Hoffmann, N. A., Hicks, C. W., & Wolberger, C. (2019). Mechanism of cross-talk between H2B ubiquitination and H3 methylation by Dot1L. Cell, 176(6), 1490-1501.

Wood, K., Tellier, M., & Murphy, S. (2018). DOT1L and H3K79 methylation in transcription and genomic stability. Biomolecules, 8(1), 11.

Zhu, B., Chen, S., Wang, H., Yin, C., Han, C., Peng, C., ... & Cui, R. (2018). The protective role of DOT1L in UV-induced melanomagenesis. Nature communications, 9(1), 1-13.

Stein, E. M., Garcia-Manero, G., Rizzieri, D. A., Tibes, R., Berdeja, J. G., Savona, M. R., ... & Tallman, M. S. (2018). The DOT1L inhibitor pinometostat reduces H3K79 methylation and has modest clinical activity in adult acute leukemia. Blood, The Journal of the American Society of Hematology, 131(24), 2661-2669.

Monteagudo, S., Cornelis, F. M., Aznar-Lopez, C., Yibmantasiri, P., Guns, L. A., Carmeliet, P., ... & Lories, R. J. (2017). DOT1L safeguards cartilage homeostasis and protects against osteoarthritis. Nature communications, 8(1), 1-12.

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For research use only. Not intended for any clinical use.

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