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Rabbit Anti-DOT1L Recombinant Antibody (D4O2T) (CBMAB-CP0493-LY)

The product is antibody recognizes DOT1L. The antibody D4O2T immunoassay techniques such as: WB,IP.
See all DOT1L antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
D4O2T
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1155 of human DOT1L protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DOT1 Like Histone Lysine Methyltransferase
Introduction
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
Entrez Gene ID
Human84444
Mouse208266
Rat362831
Monkey710033
UniProt ID
HumanQ8TEK3
MouseQ6XZL8
RatD3ZCP0
MonkeyA0A1D5QRE5
Alternative Names
DOT1 Like Histone Lysine Methyltransferase; Histone H3-K79 Methyltransferase; Histone Methyltransferase DOT1L; Lysine N-Methyltransferase 4; DOT1-Like Protein; H3-K79-HMTase; KMT4; Histone-Lysine N-Methyltransferase; H3 Lysine-79 Specific;
Function
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones (PubMed:12123582).

Binds to DNA (PubMed:12628190).
Biological Process
Chromatin silencing at telomere Source: GO_Central
DNA damage checkpoint signaling Source: GO_Central
DNA repair Source: GO_Central
Gene expression Source: Ensembl
Histone H3-K79 methylation Source: MGI
Positive regulation of cell population proliferation Source: MGI
Positive regulation of transcription by RNA polymerase II Source: MGI
Regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Regulation of transcription regulatory region DNA binding Source: UniProtKB
Telomere organization Source: BHF-UCL
Cellular Location
Nucleus

Wu, A., Zhi, J., Tian, T., Cihan, A., Cevher, M. A., Liu, Z., ... & Yu, M. (2021). DOT1L complex regulates transcriptional initiation in human erythroleukemic cells. Proceedings of the National Academy of Sciences, 118(27).

Sarno, F., Nebbioso, A., & Altucci, L. (2020). DOT1L: a key target in normal chromatin remodelling and in mixed-lineage leukaemia treatment. Epigenetics, 15(5), 439-453.

Liu, C., Yang, Q., Zhu, Q., Lu, X., Li, M., Hou, T., ... & Zhu, W. G. (2020). CBP mediated DOT1L acetylation confers DOT1L stability and promotes cancer metastasis. Theranostics, 10(4), 1758.

Anderson, C. J., Baird, M. R., Hsu, A., Barbour, E. H., Koyama, Y., Borgnia, M. J., & McGinty, R. K. (2019). Structural basis for recognition of ubiquitylated nucleosome by Dot1L methyltransferase. Cell reports, 26(7), 1681-1690.

Godfrey, L., Crump, N. T., Thorne, R., Lau, I. J., Repapi, E., Dimou, D., ... & Milne, T. A. (2019). DOT1L inhibition reveals a distinct subset of enhancers dependent on H3K79 methylation. Nature communications, 10(1), 1-15.

Worden, E. J., Hoffmann, N. A., Hicks, C. W., & Wolberger, C. (2019). Mechanism of cross-talk between H2B ubiquitination and H3 methylation by Dot1L. Cell, 176(6), 1490-1501.

Wood, K., Tellier, M., & Murphy, S. (2018). DOT1L and H3K79 methylation in transcription and genomic stability. Biomolecules, 8(1), 11.

Zhu, B., Chen, S., Wang, H., Yin, C., Han, C., Peng, C., ... & Cui, R. (2018). The protective role of DOT1L in UV-induced melanomagenesis. Nature communications, 9(1), 1-13.

Stein, E. M., Garcia-Manero, G., Rizzieri, D. A., Tibes, R., Berdeja, J. G., Savona, M. R., ... & Tallman, M. S. (2018). The DOT1L inhibitor pinometostat reduces H3K79 methylation and has modest clinical activity in adult acute leukemia. Blood, The Journal of the American Society of Hematology, 131(24), 2661-2669.

Monteagudo, S., Cornelis, F. M., Aznar-Lopez, C., Yibmantasiri, P., Guns, L. A., Carmeliet, P., ... & Lories, R. J. (2017). DOT1L safeguards cartilage homeostasis and protects against osteoarthritis. Nature communications, 8(1), 1-12.

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For research use only. Not intended for any clinical use.

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