Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-DOT1L Recombinant Antibody (K0314) (V2LY-0425-LY807)


See all DOT1L antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
K0314
Antibody Isotype
IgG2a
Application
IHC-P, IP

Basic Information

Immunogen
E. coli-derived recombinant human DOT1L (Gly2-Lys416).
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
IHC8-25 µg/ml
IP25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
Trehalose & PBS
Preservative
None
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DOT1 Like Histone Lysine Methyltransferase
Entrez Gene ID
84444
UniProt ID
Q8TEK3
Function
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones (PubMed:12123582).

Binds to DNA (PubMed:12628190).
Biological Process
Chromatin silencing at telomere Source: GO_Central
DNA damage checkpoint signaling Source: GO_Central
DNA repair Source: GO_Central
Gene expression Source: Ensembl
Histone H3-K79 methylation Source: MGI
Positive regulation of cell population proliferation Source: MGI
Positive regulation of transcription by RNA polymerase II Source: MGI
Regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Regulation of transcription regulatory region DNA binding Source: UniProtKB
Telomere organization Source: BHF-UCL
Cellular Location
Nucleus
More Infomation

Wu, A., Zhi, J., Tian, T., Cihan, A., Cevher, M. A., Liu, Z., ... & Yu, M. (2021). DOT1L complex regulates transcriptional initiation in human erythroleukemic cells. Proceedings of the National Academy of Sciences, 118(27).

Sarno, F., Nebbioso, A., & Altucci, L. (2020). DOT1L: a key target in normal chromatin remodelling and in mixed-lineage leukaemia treatment. Epigenetics, 15(5), 439-453.

Liu, C., Yang, Q., Zhu, Q., Lu, X., Li, M., Hou, T., ... & Zhu, W. G. (2020). CBP mediated DOT1L acetylation confers DOT1L stability and promotes cancer metastasis. Theranostics, 10(4), 1758.

Anderson, C. J., Baird, M. R., Hsu, A., Barbour, E. H., Koyama, Y., Borgnia, M. J., & McGinty, R. K. (2019). Structural basis for recognition of ubiquitylated nucleosome by Dot1L methyltransferase. Cell reports, 26(7), 1681-1690.

Godfrey, L., Crump, N. T., Thorne, R., Lau, I. J., Repapi, E., Dimou, D., ... & Milne, T. A. (2019). DOT1L inhibition reveals a distinct subset of enhancers dependent on H3K79 methylation. Nature communications, 10(1), 1-15.

Worden, E. J., Hoffmann, N. A., Hicks, C. W., & Wolberger, C. (2019). Mechanism of cross-talk between H2B ubiquitination and H3 methylation by Dot1L. Cell, 176(6), 1490-1501.

Wood, K., Tellier, M., & Murphy, S. (2018). DOT1L and H3K79 methylation in transcription and genomic stability. Biomolecules, 8(1), 11.

Zhu, B., Chen, S., Wang, H., Yin, C., Han, C., Peng, C., ... & Cui, R. (2018). The protective role of DOT1L in UV-induced melanomagenesis. Nature communications, 9(1), 1-13.

Stein, E. M., Garcia-Manero, G., Rizzieri, D. A., Tibes, R., Berdeja, J. G., Savona, M. R., ... & Tallman, M. S. (2018). The DOT1L inhibitor pinometostat reduces H3K79 methylation and has modest clinical activity in adult acute leukemia. Blood, The Journal of the American Society of Hematology, 131(24), 2661-2669.

Monteagudo, S., Cornelis, F. M., Aznar-Lopez, C., Yibmantasiri, P., Guns, L. A., Carmeliet, P., ... & Lories, R. J. (2017). DOT1L safeguards cartilage homeostasis and protects against osteoarthritis. Nature communications, 8(1), 1-12.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-DOT1L Recombinant Antibody (K0314)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry